Hotspot

psaC-rps15

SSC · 108619-109576 · 958 bp

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Pi 0.111

MarkerSeek score 82.90

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: SSC
length_bp: 958
variable_sites: 278
pi: 0.111
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	ACAACCATCCCAGGGTATCA	GAAAACGACAACGACTGCTG	50.0	50.0	58.0	58.0	1161-2064 bp	0.714286	73.0
psaC-rps15_p2	GGACAAGCGGATTCACATCT	GAAAACGACAACGACTGCTG	50.0	50.0	58.0	58.0	1235-2138 bp	0.714286	72.8
psaC-rps15_p3	ACAACCATCCCAGGGTATCA	CGACAACGACTGCTGAACTA	50.0	50.0	58.0	58.0	1156-2059 bp	0.714286	72.7
psaC-rps15_p4	ACAACCATCCCAGGGTATCA	TTGGGAAAACGACAACGACT	50.0	45.0	58.0	58.0	1165-2068 bp	0.285714	52.1
psaC-rps15_p5	GGACAAGCGGATTCACATCT	TTGGGAAAACGACAACGACT	50.0	45.0	58.0	58.0	1239-2142 bp	0.285714	52.0