Hotspot

ccsA-psaC

SSC · 107585-108372 · 788 bp

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Pi 0.153

MarkerSeek score 83.00

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 788
variable_sites: 293
pi: 0.153
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	TTGGAGTTGGGACCCAAAAG	TGATACCCTGGGATGGTTGT	50.0	50.0	57.9	58.0	1157-2045 bp	1.0	91.2
ccsA-psaC_p2	TTGGAGTTGGGACCCAAAAG	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.9	58.0	1083-1971 bp	1.0	91.1
ccsA-psaC_p3	CACTAATATTAGCCCCCGCC	TGATACCCTGGGATGGTTGT	55.0	50.0	57.9	58.0	1545-1738 bp	0.857143	85.2
ccsA-psaC_p4	CACTAATATTAGCCCCCGCC	AGATGTGAATCCGCTTGTCC	55.0	50.0	57.9	58.0	1471-1664 bp	0.857143	85.2
ccsA-psaC_p5	TTGGAGTTGGGACCCAAAAG	CCGACGGATTTCTTGAGTGT	50.0	50.0	57.9	57.9	1065-1258 bp	0.857143	85.0