Hotspot

psbE-petL

LSC · 61780-62600 · 821 bp

Back to Genlisea

Pi 0.0734

MarkerSeek score 85.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 166
pi: 0.0734
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CGAAATAACTAGTTATAGTAAGCA	50.0	29.2	57.8	52.3	849-930 bp	1.0	52.0
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	CGAAATAACTAGTTATAGTAAGCA	55.0	29.2	58.2	52.3	844-925 bp	1.0	52.0
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	CGAAATAACTAGTTATAGTAAGCA	50.0	29.2	58.4	52.3	842-923 bp	1.0	52.0
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	CGAAATAACTAGTTATAGTAAGCAT	50.0	28.0	57.8	52.9	849-930 bp	1.0	52.0
psbE-petL_p5	TTCTCCTGTGCTTCCAGACA	CGAAATAACTAGTTATAGTAAGCA	50.0	29.2	58.6	52.3	843-924 bp	1.0	52.0