Hotspot

rbcL-accD

LSC · 53803-54523 · 721 bp

Back to Genlisea

Pi 0.0656

MarkerSeek score 84.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 128
pi: 0.0656
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGAATGCTACTGCGGGTAC	GGACCAAGATTGTCCGTTGA	50.0	50.0	58.0	57.8	1507-1533 bp	0.857143	84.7
rbcL-accD_p2	TTGAATGCTACTGCGGGTAC	AGGACCAAGATTGTCCGTTG	50.0	50.0	58.0	57.8	1508-1534 bp	0.857143	84.7
rbcL-accD_p3	CAAGATTGGGTCTCTCTGCC	GGACCAAGATTGTCCGTTGA	55.0	50.0	58.0	57.8	1129-1145 bp	0.714286	75.3
rbcL-accD_p4	CAAGATTGGGTCTCTCTGCC	AGGACCAAGATTGTCCGTTG	55.0	50.0	58.0	57.8	1130-1146 bp	0.714286	75.3
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	CATTACGGTAGTCACGACCC	50.0	55.0	58.0	57.8	1599-1615 bp	0.714286	69.6