Hotspot

atpH-atpI

LSC · 13410-14469 · 1060 bp

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Pi 0.0938

MarkerSeek score 80.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1060
variable_sites: 240
pi: 0.0938
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GGATGGCCAGTAGTCCAAAG	CACCTCAGGCGCATATTTCT	55.0	50.0	58.0	58.0	1726-2074 bp	0.857143	84.3
atpH-atpI_p2	GGATGGCCAGTAGTCCAAAG	CTCACCTCAGGCGCATATTT	55.0	50.0	58.0	58.0	1728-2076 bp	0.857143	84.3
atpH-atpI_p3	GGATGGCCAGTAGTCCAAAG	TCACCTCAGGCGCATATTTC	55.0	50.0	58.0	58.0	1727-2075 bp	0.857143	84.3
atpH-atpI_p4	GGATGGCCAGTAGTCCAAAG	TACGACAGTTGCTTTGGCTT	55.0	45.0	58.0	58.0	1750-2098 bp	0.857143	84.2
atpH-atpI_p5	GGATGGCCAGTAGTCCAAAG	TTTCCAAGTCCACGGACAAG	55.0	50.0	58.0	58.0	2059-2407 bp	0.857143	83.8