primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnK-UUU-rps16_p1 trnK-UUU-rps16 1 GTGTTGTTGAAATCGCTCGG GCAATTGATGTTCGAGCTCG 20 20 50.0 50.0 58.0 57.9 0.0 30.0 0.132261 4206 5030 1770-1795 0.714286 74.8 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 GTGTTGTTGAAATCGCTCGG TTTTCTTGAGCCGTACGAGG 20 20 50.0 50.0 58.0 57.9 0.0 18.8 0.124075 4206 5030 1866-1891 0.714286 74.6 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 TGAAATCGCTCGGGCTTTAA TTTTCTTGAGCCGTACGAGG 20 20 45.0 50.0 57.9 57.9 5.4 18.8 0.176617 4206 5030 1859-1879 0.285714 50.6 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 TTGAAATCGCTCGGGCTTTA TTTTCTTGAGCCGTACGAGG 20 20 45.0 50.0 57.9 57.9 5.4 18.8 0.176617 4206 5030 1860-1880 0.285714 50.6 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 TGAAATCGCTCGGGCTTTAA GCAATTGATGTTCGAGCTCG 20 20 45.0 50.0 57.9 57.9 5.4 30.0 0.184803 4206 5030 1763-1782 0.285714 50.5 trnS-GCU-trnG-UCC_p1 trnS-GCU-trnG-UCC 1 TCCACTCAGCCATCTCTCC ACCACTAAACTATACCCGCT 19 20 57.9 45.0 58.1 55.6 0.0 0.0 3.446452 8112 8819 614-773 1.000000 64.4 trnS-GCU-trnG-UCC_p2 trnS-GCU-trnG-UCC 2 GTCCACTCAGCCATCTCTCC ACCACTAAACTATACCCGCT 20 20 60.0 45.0 59.5 55.6 0.0 0.0 3.890535 8112 8819 615-774 1.000000 60.9 trnS-GCU-trnG-UCC_p3 trnS-GCU-trnG-UCC 3 GTCCACTCAGCCATCTCTC ACCACTAAACTATACCCGCT 19 20 57.9 45.0 56.9 55.6 0.0 0.0 4.412981 8112 8819 615-774 1.000000 56.7 trnS-GCU-trnG-UCC_p4 trnS-GCU-trnG-UCC 4 TCCACTCAGCCATCTCTCC TACCACTAAACTATACCCGCT 19 21 57.9 42.9 58.1 55.6 0.0 0.0 4.492944 8112 8819 615-774 1.000000 56.1 trnS-GCU-trnG-UCC_p5 trnS-GCU-trnG-UCC 5 TCCACTCAGCCATCTCTCC ACGAATCACACTTTTACCACT 19 21 57.9 38.1 58.1 55.5 0.0 0.0 4.628140 8112 8819 629-788 1.000000 55.0 trnG-UCC_p1 trnG-UCC 1 ATTAGCAATCCGGCGCTTTA TTCAGGAGCAAATGGATCGG 20 20 45.0 50.0 58.0 58.0 16.5 0.0 0.080892 8820 9623 1800-1946 0.857143 85.6 trnG-UCC_p2 trnG-UCC 2 ATTAGCAATCCGGCGCTTTA ATTGGAACTGGCGCAATTTG 20 20 45.0 45.0 58.0 57.9 16.5 10.8 0.121373 8820 9623 2129-2275 0.857143 84.9 trnG-UCC_p3 trnG-UCC 3 TGAGTGTTTTTGGCCACGTA TTCAGGAGCAAATGGATCGG 20 20 45.0 50.0 58.0 58.0 3.9 0.0 0.082504 8820 9623 1341-1400 0.714286 76.4 trnG-UCC_p4 trnG-UCC 4 CCACTCAGCCATCTCTCCTA TTCAGGAGCAAATGGATCGG 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.121856 8820 9623 1863-1924 0.714286 75.7 trnG-UCC_p5 trnG-UCC 5 TGAGTGTTTTTGGCCACGTA ATTGGAACTGGCGCAATTTG 20 20 45.0 45.0 58.0 57.9 3.9 10.8 0.122985 8820 9623 1670-1729 0.714286 74.7 atpH-atpI_p1 atpH-atpI 1 GGATGGCCAGTAGTCCAAAG CACCTCAGGCGCATATTTCT 20 20 55.0 50.0 58.0 58.0 16.8 10.8 0.085081 13410 14469 1726-2074 0.857143 84.3 atpH-atpI_p2 atpH-atpI 2 GGATGGCCAGTAGTCCAAAG CTCACCTCAGGCGCATATTT 20 20 55.0 50.0 58.0 58.0 16.8 10.8 0.085081 13410 14469 1728-2076 0.857143 84.3 atpH-atpI_p3 atpH-atpI 3 GGATGGCCAGTAGTCCAAAG TCACCTCAGGCGCATATTTC 20 20 55.0 50.0 58.0 58.0 16.8 10.8 0.090564 13410 14469 1727-2075 0.857143 84.3 atpH-atpI_p4 atpH-atpI 4 GGATGGCCAGTAGTCCAAAG TACGACAGTTGCTTTGGCTT 20 20 55.0 45.0 58.0 58.0 16.8 0.0 0.082329 13410 14469 1750-2098 0.857143 84.2 atpH-atpI_p5 atpH-atpI 5 GGATGGCCAGTAGTCCAAAG TTTCCAAGTCCACGGACAAG 20 20 55.0 50.0 58.0 58.0 16.8 18.2 0.082329 13410 14469 2059-2407 0.857143 83.8 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 GCCCTTTTAACTCAGCGGTA AAAGGTTGTACCCGTGAACC 20 20 50.0 50.0 57.9 58.0 0.0 4.3 0.132056 30999 32235 1475-1498 0.857143 83.8 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 GCCCTTTTAACTCAGCGGTA TGGACCAGAGAATGCGATTG 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.131957 30999 32235 1760-1783 0.857143 82.4 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 GCCCTTTTAACTCAGCGGTA AAAAGGGTTCAGCGTCCAAT 20 20 50.0 45.0 57.9 58.0 0.0 0.0 0.108161 30999 32235 1901-1924 0.857143 82.0 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 GCCCTTTTAACTCAGCGGTA AAAGGGTTCAGCGTCCAATT 20 20 50.0 45.0 57.9 58.0 0.0 0.0 0.108161 30999 32235 1900-1923 0.857143 82.0 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 GCCCTTTTAACTCAGCGGTA GCGGTGACCATGGAATAAGT 20 20 50.0 50.0 57.9 58.0 0.0 21.5 0.141924 30999 32235 2059-2082 0.714286 70.1 psaA-ycf3_p1 psaA-ycf3 1 TGCGAGTTGGATCACTTAGC CGCGGAAGTTTGGTTTGATC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.046398 41150 42116 1241-1405 1.000000 91.6 psaA-ycf3_p2 psaA-ycf3 2 TGCGAGTTGGATCACTTAGC ATCAAGCCGCTCAGTATTGG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.051103 41150 42116 1224-1388 1.000000 91.6 psaA-ycf3_p3 psaA-ycf3 3 ACATGTACTTGATGACCCGC CGCGGAAGTTTGGTTTGATC 20 20 50.0 50.0 58.0 58.0 15.1 0.0 0.060429 41150 42116 1569-1732 0.857143 85.8 psaA-ycf3_p4 psaA-ycf3 4 ACATGTACTTGATGACCCGC ATCAAGCCGCTCAGTATTGG 20 20 50.0 50.0 58.0 58.0 15.1 0.0 0.065134 41150 42116 1552-1715 0.857143 85.8 psaA-ycf3_p5 psaA-ycf3 5 TGCGAGTTGGATCACTTAGC CAATTTGCGAGGTTTGGGTC 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.074718 41150 42116 1871-1953 0.714286 74.4 ndhK_p1 ndhK 1 AGTTGGGCGGAAATGACTTT CGTAGAGAACCAACCCTTCG 20 20 45.0 55.0 58.0 58.0 0.0 0.0 0.012454 47459 47883 1405-1474 0.714286 76.5 ndhK_p2 ndhK 2 CCTCGTGTCACCAGTTCAAA CGTAGAGAACCAACCCTTCG 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.059726 47459 47883 1111-1185 0.714286 76.1 ndhK_p3 ndhK 3 AGTTGGGCGGAAATGACTTT TGCATGGGGAAAGAAAGCAT 20 20 45.0 45.0 58.0 58.1 0.0 0.0 0.063247 47459 47883 1015-1035 0.571429 68.0 ndhK_p4 ndhK 4 GTTTCAGTAGGTGGGCCAAA CGTAGAGAACCAACCCTTCG 20 20 50.0 55.0 58.0 58.0 8.4 0.0 0.018124 47459 47883 987-992 0.285714 52.2 ndhK_p5 ndhK 5 TTCAGTAGGTGGGCCAAAAC CGTAGAGAACCAACCCTTCG 20 20 50.0 55.0 58.0 58.0 8.4 0.0 0.018124 47459 47883 985-990 0.285714 52.2 atpB-rbcL_p1 atpB-rbcL 1 ATTAGGCATCTTTCCTGGCG GTAAGTCCATCGGTCCACAC 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.033098 51567 52368 1151-1173 1.000000 91.0 atpB-rbcL_p2 atpB-rbcL 2 ATTAGGCATCTTTCCTGGCG AGAAACGATCTCTCCAACGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.029459 51567 52368 1582-1604 1.000000 90.2 atpB-rbcL_p3 atpB-rbcL 3 ATTAGGCATCTTTCCTGGCG GTACCCGCAGTAGCATTCAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.045670 51567 52368 1664-1686 1.000000 89.9 atpB-rbcL_p4 atpB-rbcL 4 GTCTAGTACCGGACCGATGA GTAAGTCCATCGGTCCACAC 20 20 55.0 55.0 58.0 58.0 17.5 0.0 0.046929 51567 52368 1122-1143 0.857143 80.7 atpB-rbcL_p5 atpB-rbcL 5 GTCTAGTACCGGACCGATGA AGAAACGATCTCTCCAACGC 20 20 55.0 50.0 58.0 58.0 17.5 0.0 0.043289 51567 52368 1553-1574 0.857143 79.6 rbcL-accD_p1 rbcL-accD 1 TTGAATGCTACTGCGGGTAC GGACCAAGATTGTCCGTTGA 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.197454 53803 54523 1507-1533 0.857143 84.7 rbcL-accD_p2 rbcL-accD 2 TTGAATGCTACTGCGGGTAC AGGACCAAGATTGTCCGTTG 20 20 50.0 50.0 58.0 57.8 0.0 3.9 0.203546 53803 54523 1508-1534 0.857143 84.7 rbcL-accD_p3 rbcL-accD 3 CAAGATTGGGTCTCTCTGCC GGACCAAGATTGTCCGTTGA 20 20 55.0 50.0 58.0 57.8 0.0 0.0 0.214442 53803 54523 1129-1145 0.714286 75.3 rbcL-accD_p4 rbcL-accD 4 CAAGATTGGGTCTCTCTGCC AGGACCAAGATTGTCCGTTG 20 20 55.0 50.0 58.0 57.8 0.0 3.9 0.220534 53803 54523 1130-1146 0.714286 75.3 rbcL-accD_p5 rbcL-accD 5 TTGAATGCTACTGCGGGTAC CATTACGGTAGTCACGACCC 20 20 50.0 55.0 58.0 57.8 0.0 0.8 0.217833 53803 54523 1599-1615 0.714286 69.6 petA-psbJ_p1 petA-psbJ 1 CAATTGGCCGAAATGAATTTCT GGATTAGGTTCATCCCTGTAG 22 21 36.4 47.6 56.2 54.9 12.6 0.0 7.927245 60039 61010 930-1057 1.000000 52.0 petA-psbJ_p2 petA-psbJ 2 CAATTGGCCGAAATGAATTTCT GGATTAGGTTCATCCCTGTA 22 20 36.4 45.0 56.2 53.5 12.6 0.0 8.314888 60039 61010 930-1057 1.000000 52.0 petA-psbJ_p3 petA-psbJ 3 ATTGGCCGAAATGAATTTCT GGATTAGGTTCATCCCTGTAG 20 21 35.0 47.6 53.8 54.9 12.6 0.0 8.325196 60039 61010 928-1055 1.000000 52.0 petA-psbJ_p4 petA-psbJ 4 CAATTGGCCGAAATGAATTTC GGATTAGGTTCATCCCTGTAG 21 21 38.1 47.6 54.7 54.9 0.0 0.0 8.407945 60039 61010 930-1057 1.000000 52.0 petA-psbJ_p5 petA-psbJ 5 AATTGGCCGAAATGAATTTCT GGATTAGGTTCATCCCTGTAG 21 21 33.3 47.6 54.7 54.9 12.6 0.0 8.445583 60039 61010 929-1056 1.000000 52.0 psbE-petL_p1 psbE-petL 1 AGAACGTTCTCCTGTGCTTC CGAAATAACTAGTTATAGTAAGCA 20 24 50.0 29.2 57.8 52.3 24.9 24.9 9.865482 61780 62600 849-930 1.000000 52.0 psbE-petL_p2 psbE-petL 2 GTTCTCCTGTGCTTCCAGAC CGAAATAACTAGTTATAGTAAGCA 20 24 55.0 29.2 58.2 52.3 0.9 24.9 9.907070 61780 62600 844-925 1.000000 52.0 psbE-petL_p3 psbE-petL 3 TCTCCTGTGCTTCCAGACAT CGAAATAACTAGTTATAGTAAGCA 20 24 50.0 29.2 58.4 52.3 0.9 24.9 10.059817 61780 62600 842-923 1.000000 52.0 psbE-petL_p4 psbE-petL 4 AGAACGTTCTCCTGTGCTTC CGAAATAACTAGTTATAGTAAGCAT 20 25 50.0 28.0 57.8 52.9 24.9 24.9 10.268888 61780 62600 849-930 1.000000 52.0 psbE-petL_p5 psbE-petL 5 TTCTCCTGTGCTTCCAGACA CGAAATAACTAGTTATAGTAAGCA 20 24 50.0 29.2 58.6 52.3 0.9 24.9 10.285555 61780 62600 843-924 1.000000 52.0 rpl20-rps12_p1 rpl20-rps12 1 ATCCGCGTTTAATCCTGGTC CGAGGAACATGTACTAGGGTG 20 21 50.0 52.4 58.1 57.6 4.9 0.0 1.488831 65670 66481 850-860 1.000000 80.0 rpl20-rps12_p2 rpl20-rps12 2 CCGCGTTTAATCCTGGTCAT CGAGGAACATGTACTAGGGTG 20 21 50.0 52.4 58.3 57.6 4.9 0.0 1.774602 65670 66481 848-858 1.000000 77.7 rpl20-rps12_p3 rpl20-rps12 3 ATCCGCGTTTAATCCTGGTC CGAGGAACATGTACTAGGGT 20 20 50.0 50.0 58.1 56.1 4.9 0.0 1.984034 65670 66481 850-860 1.000000 76.1 rpl20-rps12_p4 rpl20-rps12 4 CCGCGTTTAATCCTGGTCAT CGAGGAACATGTACTAGGGT 20 20 50.0 50.0 58.3 56.1 4.9 0.0 2.269805 65670 66481 848-858 1.000000 73.8 rpl20-rps12_p5 rpl20-rps12 5 TATCCGCGTTTAATCCTGGTC CGAGGAACATGTACTAGGGTG 21 21 47.6 52.4 57.9 57.6 4.9 0.0 2.540348 65670 66481 851-861 1.000000 71.6 rpl16_p1 rpl16 1 ACACATCAATTCTCGAGCCC TCCGCTACAAACCATTCGAG 20 20 50.0 50.0 58.0 58.0 7.8 0.0 0.034421 77851 79126 1674-1716 0.857143 84.8 rpl16_p2 rpl16 2 AACGGGTCTGTGGTTGAATC TCCGCTACAAACCATTCGAG 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.112373 77851 79126 1633-1675 0.857143 84.3 rpl16_p3 rpl16 3 CCCCAACCCCAAAAAGATCA TCCGCTACAAACCATTCGAG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.027300 77851 79126 2017-2059 0.571429 68.5 rpl16_p4 rpl16 4 ACCCCAACCCCAAAAAGATC TCCGCTACAAACCATTCGAG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.028758 77851 79126 2018-2060 0.571429 68.5 rpl16_p5 rpl16 5 CCCCAACCCCAAAAAGATCA CTTGCCGAATTTTTAGCCGG 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.114652 77851 79126 2190-2232 0.571429 67.4 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 ACAACTCTTTGGCAGCGTTA GGATTCGAACCGAGATGCTT 20 20 45.0 50.0 58.0 58.1 0.0 0.0 0.096882 105941 106456 632-711 0.571429 74.1 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 AATCCAGTCGGTCATTGTGG TTCGAACCGAGATGCTTGAG 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.117569 105941 106456 1217-1394 0.571429 73.9 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 ACAACTCTTTGGCAGCGTTA TTCGAACCGAGATGCTTGAG 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.041915 105941 106456 629-708 0.428571 68.2 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 TCGCTTTCTACGGGTAATGC TTCGAACCGAGATGCTTGAG 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.073141 105941 106456 642-667 0.285714 54.5 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 TCGCTTTCTACGGGTAATGC GGATTCGAACCGAGATGCTT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.128108 105941 106456 645-670 0.285714 54.1 ccsA-psaC_p1 ccsA-psaC 1 TTGGAGTTGGGACCCAAAAG TGATACCCTGGGATGGTTGT 20 20 50.0 50.0 57.9 58.0 27.9 22.2 0.100845 107585 108372 1157-2045 1.000000 91.2 ccsA-psaC_p2 ccsA-psaC 2 TTGGAGTTGGGACCCAAAAG AGATGTGAATCCGCTTGTCC 20 20 50.0 50.0 57.9 58.0 27.9 0.0 0.110492 107585 108372 1083-1971 1.000000 91.1 ccsA-psaC_p3 ccsA-psaC 3 CACTAATATTAGCCCCCGCC TGATACCCTGGGATGGTTGT 20 20 55.0 50.0 57.9 58.0 20.2 22.2 0.129615 107585 108372 1545-1738 0.857143 85.2 ccsA-psaC_p4 ccsA-psaC 4 CACTAATATTAGCCCCCGCC AGATGTGAATCCGCTTGTCC 20 20 55.0 50.0 57.9 58.0 20.2 0.0 0.139263 107585 108372 1471-1664 0.857143 85.2 ccsA-psaC_p5 ccsA-psaC 5 TTGGAGTTGGGACCCAAAAG CCGACGGATTTCTTGAGTGT 20 20 50.0 50.0 57.9 57.9 27.9 8.6 0.160940 107585 108372 1065-1258 0.857143 85.0 psaC-rps15_p1 psaC-rps15 1 ACAACCATCCCAGGGTATCA GAAAACGACAACGACTGCTG 20 20 50.0 50.0 58.0 58.0 5.0 0.0 0.025379 108619 109576 1161-2064 0.714286 73.0 psaC-rps15_p2 psaC-rps15 2 GGACAAGCGGATTCACATCT GAAAACGACAACGACTGCTG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.035027 108619 109576 1235-2138 0.714286 72.8 psaC-rps15_p3 psaC-rps15 3 ACAACCATCCCAGGGTATCA CGACAACGACTGCTGAACTA 20 20 50.0 50.0 58.0 58.0 5.0 0.0 0.066473 108619 109576 1156-2059 0.714286 72.7 psaC-rps15_p4 psaC-rps15 4 ACAACCATCCCAGGGTATCA TTGGGAAAACGACAACGACT 20 20 50.0 45.0 58.0 58.0 5.0 0.0 0.047194 108619 109576 1165-2068 0.285714 52.1 psaC-rps15_p5 psaC-rps15 5 GGACAAGCGGATTCACATCT TTGGGAAAACGACAACGACT 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.056842 108619 109576 1239-2142 0.285714 52.0 rps15-ycf1_p1 rps15-ycf1 1 CAGCAGTCGTTGTCGTTTTC TGCAACGGAAAGAGTGTCAA 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.036447 109844 110225 789-790 0.285714 52.7 rps15-ycf1_p2 rps15-ycf1 2 CAGCAGTCGTTGTCGTTTTC TTGCAACGGAAAGAGTGTCA 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.036447 109844 110225 790-791 0.285714 52.7 rps15-ycf1_p3 rps15-ycf1 3 AGTCGTTGTCGTTTTCCCAA TGCAACGGAAAGAGTGTCAA 20 20 45.0 45.0 58.0 58.0 0.0 0.0 0.058262 109844 110225 785-786 0.285714 52.5 rps15-ycf1_p4 rps15-ycf1 4 AGTCGTTGTCGTTTTCCCAA TTGCAACGGAAAGAGTGTCA 20 20 45.0 45.0 58.0 58.0 0.0 0.0 0.058262 109844 110225 786-787 0.285714 52.5 rps15-ycf1_p5 rps15-ycf1 5 TAGTTCAGCAGTCGTTGTCG TGCAACGGAAAGAGTGTCAA 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.077541 109844 110225 794-795 0.285714 52.3 ycf1_p1 ycf1 1 CATCCCCGTTTGATATGCCA TCCGAGCTCAGGTTATGGAA 20 20 50.0 50.0 58.0 58.1 0.0 15.4 0.159504 114727 114846 1008-1050 0.714286 75.3 ycf1_p2 ycf1 2 CATCCCCGTTTGATATGCCA AAGGTATCAGCAACAACCGG 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.198929 114727 114846 973-1015 0.714286 75.1 ycf1_p3 ycf1 3 CATCCCCGTTTGATATGCCA AATCCAGTCGGTCATTGTGG 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.134006 114727 114846 1088-1130 0.714286 74.9 ycf1_p4 ycf1 4 GACCCCTTCTTCCACGTTTT TCCGAGCTCAGGTTATGGAA 20 20 50.0 50.0 58.0 58.1 0.0 15.4 0.162303 114727 114846 641-668 0.714286 74.8 ycf1_p5 ycf1 5 GACCCCTTCTTCCACGTTTT AATCCAGTCGGTCATTGTGG 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.136804 114727 114846 721-748 0.714286 74.2