Hotspot

atpF

LSC · 61197-62485 · 1289 bp

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Pi 0.0044

MarkerSeek score 62.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1289
variable_sites: 8
pi: 0.0044
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGCACTTTTATTTGCGAATCCT	CACGAATGCTTACCATGAGT	36.4	45.0	56.9	55.9	1463-1470 bp	1.0	40.9
atpF_p2	AGCATTAGCACTTTTATTTGCGA	CACGAATGCTTACCATGAGT	34.8	45.0	57.7	55.9	1469-1476 bp	1.0	40.9
atpF_p3	AGCACTTTTATTTGCGAATCCTT	CACGAATGCTTACCATGAGT	34.8	45.0	57.6	55.9	1463-1470 bp	1.0	40.9
atpF_p4	AGCACTTTTATTTGCGAATCC	CACGAATGCTTACCATGAGT	38.1	45.0	55.5	55.9	1463-1470 bp	1.0	40.9
atpF_p5	GCACTTTTATTTGCGAATCCT	CACGAATGCTTACCATGAGT	38.1	45.0	55.5	55.9	1462-1469 bp	1.0	40.9