Hotspot

rpl32-ndhF

SSC · 123556-124305 · 750 bp

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Pi 0.016

MarkerSeek score 58.70

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 750
variable_sites: 18
pi: 0.016
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TCCTAAGAGCAGCGTGTCTA	GTGGGAAAAAGTAGGAGCCC	50.0	55.0	57.9	58.2	1335-1353 bp	1.0	80.8
rpl32-ndhF_p2	TCCTAAGAGCAGCGTGTCTA	GGAACCGGAAGTGGAATGAA	50.0	50.0	57.9	57.8	1299-1317 bp	1.0	80.6
rpl32-ndhF_p3	TCCTAAGAGCAGCGTGTCTA	GCAATAGGACGTGTGAACCA	50.0	50.0	57.9	58.2	1755-1773 bp	1.0	79.9
rpl32-ndhF_p4	TCCTAAGAGCAGCGTGTCTA	GGACGTGTGAACCAAAAACC	50.0	50.0	57.9	57.8	1749-1767 bp	1.0	79.9
rpl32-ndhF_p5	TCCTAAGAGCAGCGTGTCTA	GGAAAAAGTAGGAGCCCCAC	50.0	55.0	57.9	58.2	1332-1342 bp	0.666667	67.3