Hotspot

ycf1-rps15

SSC · 113290-113718 · 429 bp

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Pi 0.0241

MarkerSeek score 64.60

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 429
variable_sites: 15
pi: 0.0241
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TCCTCGATTGAAGATTCGTT	TTTTTCTTCGGAAATAATTGGT	40.0	27.3	54.5	52.5	481-493 bp	1.0	45.9
ycf1-rps15_p2	ATCCTCGATTGAAGATTCGT	TTTTTCTTCGGAAATAATTGGT	40.0	27.3	54.3	52.5	482-494 bp	1.0	45.9
ycf1-rps15_p3	ATCCTCGATTGAAGATTCGTT	TTTTTCTTCGGAAATAATTGGT	38.1	27.3	55.1	52.5	482-494 bp	1.0	45.9
ycf1-rps15_p4	TGTATCCTCGATTGAAGATTCGT	TTTTTCTTCGGAAATAATTGGT	39.1	27.3	57.5	52.5	485-497 bp	1.0	45.8
ycf1-rps15_p5	ATGTATCCTCGATTGAAGATTCGT	TTTTTCTTCGGAAATAATTGGT	37.5	27.3	57.9	52.5	486-498 bp	1.0	45.8