Hotspot

trnF-ndhJ

LSC · 69426-70020 · 595 bp

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Pi 0.0202

MarkerSeek score 63.90

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 595
variable_sites: 18
pi: 0.0202
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TTGGATAGGATGGCCTTTGC	55.0	50.0	58.0	57.9	735-852 bp	1.0	81.3
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTGC	50.0	50.0	58.1	57.9	699-816 bp	1.0	81.0
trnF-ndhJ_p3	AGAATGGTCGGGATAGCTCA	TTGGATAGGATGGCCTTTGC	50.0	50.0	57.9	57.9	744-861 bp	1.0	80.6
trnF-ndhJ_p4	CCAAGAATGGTCGGGATAGC	TTGGATAGGATGGCCTTTGC	55.0	50.0	58.1	57.9	747-864 bp	1.0	80.3
trnF-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	TTGGATAGGATGGCCTTTGC	55.0	50.0	57.9	57.9	723-840 bp	1.0	80.2