Hotspot

trnG-trnS

LSC · 65612-66450 · 839 bp

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Pi 0.0115

MarkerSeek score 60.60

Primer available yes

Label trnG-trnS

Feature Metadata

label_name: trnG-trnS
feature_type: igs
region: LSC
length_bp: 839
variable_sites: 14
pi: 0.0115
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnS_p1	TGAATCAAACGAGGGATCCC	CAATCCGACGCTTTAGTCCA	50.0	50.0	57.3	58.0	747-953 bp	1.0	79.6
trnG-trnS_p2	TGAATCAAACGAGGGATCCC	AATCCGACGCTTTAGTCCAC	50.0	50.0	57.3	58.0	746-952 bp	1.0	79.5
trnG-trnS_p3	TGAATCAAACGAGGGATCCC	AGCAATCCGACGCTTTAGTC	50.0	50.0	57.3	58.4	749-955 bp	1.0	76.7
trnG-trnS_p4	TGAATCAAACGAGGGATCCC	CGACGCTTTAGTCCACTCAG	50.0	55.0	57.3	58.4	742-948 bp	1.0	76.5
trnG-trnS_p5	TGAATCAAACGAGGGATCCC	GCTTTAGTCCACTCAGCCAT	50.0	50.0	57.3	57.6	738-944 bp	1.0	76.3