Hotspot

rps12-rpl20

LSC · 14818-15558 · 741 bp

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Pi 0.0063
MarkerSeek score 62.80
Primer available yes
Label rps12-rpl20

Feature Metadata

label_name
rps12-rpl20
feature_type
igs
region
LSC
length_bp
741
variable_sites
7
pi
0.0063
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-rpl20_p1 ACAGCCAATCCGAAATGTCA TTTTGTCCTCCGTTTCCGAG 45.0 50.0 57.8 58.1 868-893 bp 1.0 78.0
rps12-rpl20_p2 ACAAGACAGCCAATCCGAAA TTTTGTCCTCCGTTTCCGAG 45.0 50.0 57.7 58.1 873-898 bp 1.0 77.5
rps12-rpl20_p3 AGGAACGTGTACTAGGGTGT TTTTGTCCTCCGTTTCCGAG 50.0 50.0 57.7 58.1 811-836 bp 1.0 77.3
rps12-rpl20_p4 ACAGCCAATCCGAAATGTCA TTTTTGTCCTCCGTTTCCGA 45.0 45.0 57.8 57.7 869-894 bp 1.0 76.4
rps12-rpl20_p5 ACAAGACAGCCAATCCGAAA TTTTTGTCCTCCGTTTCCGA 45.0 45.0 57.7 57.7 874-899 bp 1.0 75.8