primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbB-clpP_p1	psbB-clpP	1	AAACGGCCGGGATCATTTAA	CTTTTGGAACACCAATGGGC	20	20	45.0	50.0	57.9	57.8	31.8	3.6	0.300102	12053	12523	548-549	1.000000	80.8
psbB-clpP_p2	psbB-clpP	2	AACGGCCGGGATCATTTAAA	CTTTTGGAACACCAATGGGC	20	20	45.0	50.0	57.9	57.8	31.8	3.6	0.300102	12053	12523	547-548	1.000000	80.8
psbB-clpP_p3	psbB-clpP	3	ACGGCCGGGATCATTTAAAA	CTTTTGGAACACCAATGGGC	20	20	45.0	50.0	57.9	57.8	31.8	3.6	0.300102	12053	12523	546-547	1.000000	80.8
psbB-clpP_p4	psbB-clpP	4	AACGGCCGGGATCATTTAAA	TTTGGAACACCAATGGGCAT	20	20	45.0	45.0	57.9	58.3	31.8	3.6	0.404714	12053	12523	545-546	1.000000	80.0
psbB-clpP_p5	psbB-clpP	5	ACGGCCGGGATCATTTAAAA	TTTGGAACACCAATGGGCAT	20	20	45.0	45.0	57.9	58.3	31.8	3.6	0.404714	12053	12523	544-545	1.000000	80.0
rps12-rpl20_p1	rps12-rpl20	1	ACAGCCAATCCGAAATGTCA	TTTTGTCCTCCGTTTCCGAG	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.325129	14818	15558	868-893	1.000000	78.0
rps12-rpl20_p2	rps12-rpl20	2	ACAAGACAGCCAATCCGAAA	TTTTGTCCTCCGTTTCCGAG	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.396654	14818	15558	873-898	1.000000	77.5
rps12-rpl20_p3	rps12-rpl20	3	AGGAACGTGTACTAGGGTGT	TTTTGTCCTCCGTTTCCGAG	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.425628	14818	15558	811-836	1.000000	77.3
rps12-rpl20_p4	rps12-rpl20	4	ACAGCCAATCCGAAATGTCA	TTTTTGTCCTCCGTTTCCGA	20	20	45.0	45.0	57.8	57.7	0.0	0.0	0.527646	14818	15558	869-894	1.000000	76.4
rps12-rpl20_p5	rps12-rpl20	5	ACAAGACAGCCAATCCGAAA	TTTTTGTCCTCCGTTTCCGA	20	20	45.0	45.0	57.7	57.7	0.0	0.0	0.599171	14818	15558	874-899	1.000000	75.8
trnY-trnD_p1	trnY-trnD	1	TGGCAATATGTCTACGCTGG	TATCTATCGGGACTGACGGG	20	20	50.0	55.0	57.8	57.8	0.0	0.0	0.434683	43962	44375	525-535	1.000000	80.3
trnY-trnD_p2	trnY-trnD	2	GGCAATATGTCTACGCTGGT	TATCTATCGGGACTGACGGG	20	20	50.0	55.0	57.8	57.8	0.0	0.0	0.436235	43962	44375	524-534	1.000000	80.3
trnY-trnD_p3	trnY-trnD	3	GGGGACGGACTGTAAATTCG	TATCTATCGGGACTGACGGG	20	20	55.0	55.0	58.4	57.8	0.0	0.0	0.547237	43962	44375	546-556	1.000000	79.2
trnY-trnD_p4	trnY-trnD	4	GGGACGGACTGTAAATTCGT	TATCTATCGGGACTGACGGG	20	20	50.0	55.0	57.6	57.8	0.0	0.0	0.570914	43962	44375	545-555	1.000000	79.1
trnY-trnD_p5	trnY-trnD	5	TGGCAATATGTCTACGCTGG	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.8	58.5	0.0	7.1	0.716274	43962	44375	474-484	1.000000	78.4
rpoC2-rps2_p1	rpoC2-rps2	1	TGTTCCACCACAGAGAAGTT	AATGAACTCCCGCTTCCATC	20	20	45.0	50.0	56.6	58.0	0.0	0.0	1.439156	57596	57799	324-327	1.000000	70.9
rpoC2-rps2_p2	rpoC2-rps2	2	TTGTTCCACCACAGAGAAGT	AATGAACTCCCGCTTCCATC	20	20	45.0	50.0	56.6	58.0	0.0	0.0	1.439156	57596	57799	325-328	1.000000	70.9
rpoC2-rps2_p3	rpoC2-rps2	3	TGTTCCACCACAGAGAAGTT	CCCGCTTCCATCATCTCTTC	20	20	45.0	55.0	56.6	58.1	0.0	0.0	1.518711	57596	57799	316-319	1.000000	70.4
rpoC2-rps2_p4	rpoC2-rps2	4	TGTTCCACCACAGAGAAGTT	CCGCTTCCATCATCTCTTCC	20	20	45.0	55.0	56.6	58.1	0.0	0.0	1.518711	57596	57799	315-318	1.000000	70.4
rpoC2-rps2_p5	rpoC2-rps2	5	TTGTTCCACCACAGAGAAGT	CCGCTTCCATCATCTCTTCC	20	20	45.0	55.0	56.6	58.1	0.0	0.0	1.518711	57596	57799	316-319	1.000000	70.4
atpF_p1	atpF	1	AGCACTTTTATTTGCGAATCCT	CACGAATGCTTACCATGAGT	22	20	36.4	45.0	56.9	55.9	1.3	0.0	5.202261	61197	62485	1463-1470	1.000000	40.9
atpF_p2	atpF	2	AGCATTAGCACTTTTATTTGCGA	CACGAATGCTTACCATGAGT	23	20	34.8	45.0	57.7	55.9	0.0	0.0	5.457355	61197	62485	1469-1476	1.000000	40.9
atpF_p3	atpF	3	AGCACTTTTATTTGCGAATCCTT	CACGAATGCTTACCATGAGT	23	20	34.8	45.0	57.6	55.9	1.3	0.0	5.539345	61197	62485	1463-1470	1.000000	40.9
atpF_p4	atpF	4	AGCACTTTTATTTGCGAATCC	CACGAATGCTTACCATGAGT	21	20	38.1	45.0	55.5	55.9	1.3	0.0	5.673008	61197	62485	1463-1470	1.000000	40.9
atpF_p5	atpF	5	GCACTTTTATTTGCGAATCCT	CACGAATGCTTACCATGAGT	21	20	38.1	45.0	55.5	55.9	1.3	0.0	5.673008	61197	62485	1462-1469	1.000000	40.9
trnG-trnS_p1	trnG-trnS	1	TGAATCAAACGAGGGATCCC	CAATCCGACGCTTTAGTCCA	20	20	50.0	50.0	57.3	58.0	24.6	0.0	0.714678	65612	66450	747-953	1.000000	79.6
trnG-trnS_p2	trnG-trnS	2	TGAATCAAACGAGGGATCCC	AATCCGACGCTTTAGTCCAC	20	20	50.0	50.0	57.3	58.0	24.6	0.0	0.716066	65612	66450	746-952	1.000000	79.5
trnG-trnS_p3	trnG-trnS	3	TGAATCAAACGAGGGATCCC	AGCAATCCGACGCTTTAGTC	20	20	50.0	50.0	57.3	58.4	24.6	0.0	1.068519	65612	66450	749-955	1.000000	76.7
trnG-trnS_p4	trnG-trnS	4	TGAATCAAACGAGGGATCCC	CGACGCTTTAGTCCACTCAG	20	20	50.0	55.0	57.3	58.4	24.6	0.0	1.091235	65612	66450	742-948	1.000000	76.5
trnG-trnS_p5	trnG-trnS	5	TGAATCAAACGAGGGATCCC	GCTTTAGTCCACTCAGCCAT	20	20	50.0	50.0	57.3	57.6	24.6	0.9	1.120044	65612	66450	738-944	1.000000	76.3
rps4-trnT_p1	rps4-trnT	1	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.3	57.9	9.5	0.0	0.409520	67359	67628	359-360	1.000000	80.1
rps4-trnT_p2	rps4-trnT	2	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.3	57.9	14.7	0.0	0.398577	67359	67628	381-382	1.000000	80.0
rps4-trnT_p3	rps4-trnT	3	GAAACGAGGCCCTCTGTAAC	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	58.3	57.8	9.5	0.0	0.517274	67359	67628	327-328	1.000000	79.5
rps4-trnT_p4	rps4-trnT	4	TTTTTGAAACGAGGCCCTCT	CTCAGAGGTTAGAGCATCGC	20	20	45.0	55.0	57.6	57.9	9.5	0.0	0.491062	67359	67628	364-365	1.000000	79.4
rps4-trnT_p5	rps4-trnT	5	CTCCCAGACGGCGTATTTTT	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.3	57.8	14.7	0.0	0.506331	67359	67628	349-350	1.000000	79.4
trnT-trnL_p1	trnT-trnL	1	CGAAGCCATACCCAATCGAA	CCAACGCGAAAGGAAATGTC	20	20	50.0	50.0	58.1	58.0	0.0	15.9	0.088149	67702	68376	1544-1576	1.000000	83.3
trnT-trnL_p2	trnT-trnL	2	AAAACCTCACCTGTTGACCC	TAACCCAACGCGAAAGGAAA	20	20	50.0	45.0	57.9	58.0	0.0	15.9	0.102813	67702	68376	1490-1522	1.000000	83.3
trnT-trnL_p3	trnT-trnL	3	AAAACCTCACCTGTTGACCC	TTTCCTAACCCAACGCGAAA	20	20	50.0	45.0	57.9	58.0	0.0	15.9	0.102813	67702	68376	1495-1527	1.000000	83.3
trnT-trnL_p4	trnT-trnL	4	CGAAGCCATACCCAATCGAA	TAACCCAACGCGAAAGGAAA	20	20	50.0	45.0	58.1	58.0	0.0	15.9	0.087343	67702	68376	1548-1580	1.000000	83.2
trnT-trnL_p5	trnT-trnL	5	CGAAGCCATACCCAATCGAA	TTTCCTAACCCAACGCGAAA	20	20	50.0	45.0	58.1	58.0	0.0	15.9	0.087343	67702	68376	1553-1585	1.000000	83.2
trnL-trnF_p1	trnL-trnF	1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	57.5	58.0	0.0	17.4	0.476915	68955	69352	448-452	1.000000	79.2
trnL-trnF_p2	trnL-trnF	2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	68955	69352	484-488	1.000000	78.6
trnL-trnF_p3	trnL-trnF	3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.564710	68955	69352	499-503	1.000000	78.2
trnL-trnF_p4	trnL-trnF	4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	20	20	50.0	55.0	57.5	57.9	0.0	0.0	0.610708	68955	69352	460-464	1.000000	78.1
trnL-trnF_p5	trnL-trnF	5	AATCGTGAGGGTTCAAGTCC	CATGTGCCAGGAACCAGATT	20	20	50.0	50.0	57.5	58.2	0.0	0.0	0.631042	68955	69352	502-506	1.000000	77.6
trnF-ndhJ_p1	trnF-ndhJ	1	GGGATAGCTCAGCTGGTAGA	TTGGATAGGATGGCCTTTGC	20	20	55.0	50.0	58.0	57.9	10.3	3.3	0.074283	69426	70020	735-852	1.000000	81.3
trnF-ndhJ_p2	trnF-ndhJ	2	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTGC	20	20	50.0	50.0	58.1	57.9	0.0	3.3	0.123673	69426	70020	699-816	1.000000	81.0
trnF-ndhJ_p3	trnF-ndhJ	3	AGAATGGTCGGGATAGCTCA	TTGGATAGGATGGCCTTTGC	20	20	50.0	50.0	57.9	57.9	0.0	3.3	0.159882	69426	70020	744-861	1.000000	80.6
trnF-ndhJ_p4	trnF-ndhJ	4	CCAAGAATGGTCGGGATAGC	TTGGATAGGATGGCCTTTGC	20	20	55.0	50.0	58.1	57.9	0.0	3.3	0.187542	69426	70020	747-864	1.000000	80.3
trnF-ndhJ_p5	trnF-ndhJ	5	CTGGTAGAGCAGAGGACTGA	TTGGATAGGATGGCCTTTGC	20	20	55.0	50.0	57.9	57.9	2.5	3.3	0.208076	69426	70020	723-840	1.000000	80.2
ndhC-trnV_p1	ndhC-trnV	1	TCATATTCGTAAACCAAAAACA	AAAAGATGCACGTTGGGTCT	22	20	27.3	45.0	52.3	58.0	0.0	0.0	7.675438	71698	72152	548-582	1.000000	46.1
ndhC-trnV_p2	ndhC-trnV	2	TCATATTCGTAAACCAAAAACA	AAAGATGCACGTTGGGTCTT	22	20	27.3	45.0	52.3	58.0	0.0	0.0	7.675438	71698	72152	547-581	1.000000	46.1
ndhC-trnV_p3	ndhC-trnV	3	TCATATTCGTAAACCAAAAACA	AAGATGCACGTTGGGTCTTT	22	20	27.3	45.0	52.3	58.0	0.0	0.0	7.675438	71698	72152	546-580	1.000000	46.1
ndhC-trnV_p4	ndhC-trnV	4	TCATATTCGTAAACCAAAAACAT	AAAGATGCACGTTGGGTCTT	23	20	26.1	45.0	53.0	58.0	0.0	0.0	8.026048	71698	72152	547-581	1.000000	46.1
ndhC-trnV_p5	ndhC-trnV	5	TCATATTCGTAAACCAAAAACAT	AAGATGCACGTTGGGTCTTT	23	20	26.1	45.0	53.0	58.0	0.0	0.0	8.026048	71698	72152	546-580	1.000000	46.1
rbcL-trnK_p1	rbcL-trnK	1	AGTCCTGAATTAGCTGCTGC	AAAGCCGAGTACTCTACCGT	20	20	50.0	50.0	58.0	58.2	13.4	20.3	0.203950	77328	78180	897-972	1.000000	84.1
rbcL-trnK_p2	rbcL-trnK	2	AGTCCTGAATTAGCTGCTGC	AAGCCGAGTACTCTACCGTT	20	20	50.0	50.0	58.0	58.2	13.4	20.3	0.203950	77328	78180	896-971	1.000000	84.1
rbcL-trnK_p3	rbcL-trnK	3	GTGAGGCTTCCAAATGGAGT	AAGCCGAGTACTCTACCGTT	20	20	50.0	50.0	57.8	58.2	1.9	20.3	0.374996	77328	78180	913-988	1.000000	82.8
rbcL-trnK_p4	rbcL-trnK	4	GTGAGGCTTCCAAATGGAGT	AAAGCCGAGTACTCTACCGT	20	20	50.0	50.0	57.8	58.2	1.9	20.3	0.374996	77328	78180	914-989	1.000000	82.7
rbcL-trnK_p5	rbcL-trnK	5	GAGGCTTCCAAATGGAGTCC	AAGCCGAGTACTCTACCGTT	20	20	55.0	50.0	58.2	58.2	0.7	20.3	0.411990	77328	78180	911-986	1.000000	82.5
psbA-trnH_p1	psbA-trnH	1	TTCCCTCTAGACCTAGCTGC	GATCCACTTGGCTACATCCG	20	20	55.0	55.0	57.9	58.1	0.0	0.0	0.203648	82222	82565	322-414	1.000000	85.2
psbA-trnH_p2	psbA-trnH	2	TTCCCTCTAGACCTAGCTGC	CAATCCACTGCCTTGATCCA	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.208010	82222	82565	336-428	1.000000	85.0
psbA-trnH_p3	psbA-trnH	3	TTCCCTCTAGACCTAGCTGC	AATCCACTGCCTTGATCCAC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.209518	82222	82565	335-427	1.000000	85.0
psbA-trnH_p4	psbA-trnH	4	TTCCCTCTAGACCTAGCTGC	ACAATCCACTGCCTTGATCC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.209518	82222	82565	337-429	1.000000	85.0
psbA-trnH_p5	psbA-trnH	5	TTCCCTCTAGACCTAGCTGC	ATTCATTTCTGGGCGAACGA	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.182679	82222	82565	386-478	1.000000	84.9
trnH-rpl2_p1	trnH-rpl2	1	CGGATGTAGCCAAGTGGATC	TGCGTCGCCGTAGTAAATAG	20	20	55.0	50.0	58.1	57.9	0.2	15.9	0.238528	82641	82755	207-215	1.000000	80.0
trnH-rpl2_p2	trnH-rpl2	2	TGGATCAAGGCAGTGGATTG	GGGCCCCAATTGGTAGAAAA	20	20	50.0	50.0	57.9	58.1	0.0	22.9	0.187702	82641	82755	292-300	1.000000	79.9
trnH-rpl2_p3	trnH-rpl2	3	CGGATGTAGCCAAGTGGATC	GGGCCCCAATTGGTAGAAAA	20	20	55.0	50.0	58.1	58.1	0.2	22.9	0.183340	82641	82755	306-314	1.000000	79.8
trnH-rpl2_p4	trnH-rpl2	4	GGATCAAGGCAGTGGATTGT	GGGCCCCAATTGGTAGAAAA	20	20	50.0	50.0	57.9	58.1	0.0	22.9	0.189209	82641	82755	291-299	1.000000	79.8
trnH-rpl2_p5	trnH-rpl2	5	GTGGATCAAGGCAGTGGATT	GGGCCCCAATTGGTAGAAAA	20	20	50.0	50.0	57.9	58.1	0.0	22.9	0.189209	82641	82755	293-301	1.000000	79.8
ycf1_p1	ycf1	1	TCAATGAACGTCATGTGGCT	GGATTGGTATGATCCACGGG	20	20	45.0	55.0	57.8	57.8	0.0	0.0	0.368482	111872	111991	474-501	1.000000	83.4
ycf1_p2	ycf1	2	TCAATGAACGTCATGTGGCT	TTTGGCTTGGTTTCGAGTGT	20	20	45.0	45.0	57.8	58.2	0.0	0.0	0.425796	111872	111991	620-647	1.000000	82.2
ycf1_p3	ycf1	3	TGCGGAAAGGGCTGATAAAA	GGATTGGTATGATCCACGGG	20	20	45.0	55.0	57.8	57.8	0.0	0.0	0.393136	111872	111991	875-902	1.000000	81.9
ycf1_p4	ycf1	4	TCAATGAACGTCATGTGGCT	TGTCTTGTACGATTCTGCCG	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.192679	111872	111991	719-737	0.666667	67.2
ycf1_p5	ycf1	5	TGCGGAAAGGGCTGATAAAA	TGTCTTGTACGATTCTGCCG	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.217333	111872	111991	1120-1138	0.666667	66.3
ycf1-rps15_p1	ycf1-rps15	1	TCCTCGATTGAAGATTCGTT	TTTTTCTTCGGAAATAATTGGT	20	22	40.0	27.3	54.5	52.5	0.0	0.0	10.940600	113290	113718	481-493	1.000000	45.9
ycf1-rps15_p2	ycf1-rps15	2	ATCCTCGATTGAAGATTCGT	TTTTTCTTCGGAAATAATTGGT	20	22	40.0	27.3	54.3	52.5	0.0	0.0	11.178622	113290	113718	482-494	1.000000	45.9
ycf1-rps15_p3	ycf1-rps15	3	ATCCTCGATTGAAGATTCGTT	TTTTTCTTCGGAAATAATTGGT	21	22	38.1	27.3	55.1	52.5	0.0	0.0	11.328193	113290	113718	482-494	1.000000	45.9
ycf1-rps15_p4	ycf1-rps15	4	TGTATCCTCGATTGAAGATTCGT	TTTTTCTTCGGAAATAATTGGT	23	22	39.1	27.3	57.5	52.5	0.0	0.0	10.969978	113290	113718	485-497	1.000000	45.8
ycf1-rps15_p5	ycf1-rps15	5	ATGTATCCTCGATTGAAGATTCGT	TTTTTCTTCGGAAATAATTGGT	24	22	37.5	27.3	57.9	52.5	0.0	0.0	11.544664	113290	113718	486-498	1.000000	45.8
ndhI-ndhG_p1	ndhI-ndhG	1	AATCAAATTGCTTTGGGCCG	ATGTAGCGGTTCAGGCAAAT	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.226450	118098	118458	460-484	1.000000	81.9
ndhI-ndhG_p2	ndhI-ndhG	2	TTTGGGCCGTTTACCAATGT	ATGTAGCGGTTCAGGCAAAT	20	20	45.0	45.0	58.3	57.9	0.0	0.0	0.422898	118098	118458	449-473	1.000000	80.5
ndhI-ndhG_p3	ndhI-ndhG	3	AATCAAATTGCTTTGGGCCG	AATCATGTAGCGGTTCAGGC	20	20	45.0	50.0	57.9	58.3	0.0	0.8	0.429260	118098	118458	464-488	1.000000	80.3
ndhI-ndhG_p4	ndhI-ndhG	4	CTTTGGGCCGTTTACCAATG	ATGTAGCGGTTCAGGCAAAT	20	20	50.0	45.0	57.6	57.9	0.0	0.0	0.490940	118098	118458	450-474	1.000000	79.9
ndhI-ndhG_p5	ndhI-ndhG	5	CAAATTGCTTTGGGCCGTTT	ATGTAGCGGTTCAGGCAAAT	20	20	45.0	45.0	58.4	57.9	0.0	0.0	0.536707	118098	118458	457-481	1.000000	79.5
rpl32-ndhF_p1	rpl32-ndhF	1	TCCTAAGAGCAGCGTGTCTA	GTGGGAAAAAGTAGGAGCCC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.288783	123556	124305	1335-1353	1.000000	80.8
rpl32-ndhF_p2	rpl32-ndhF	2	TCCTAAGAGCAGCGTGTCTA	GGAACCGGAAGTGGAATGAA	20	20	50.0	50.0	57.9	57.8	0.0	9.5	0.318479	123556	124305	1299-1317	1.000000	80.6
rpl32-ndhF_p3	rpl32-ndhF	3	TCCTAAGAGCAGCGTGTCTA	GCAATAGGACGTGTGAACCA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.320943	123556	124305	1755-1773	1.000000	79.9
rpl32-ndhF_p4	rpl32-ndhF	4	TCCTAAGAGCAGCGTGTCTA	GGACGTGTGAACCAAAAACC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.322069	123556	124305	1749-1767	1.000000	79.9
rpl32-ndhF_p5	rpl32-ndhF	5	TCCTAAGAGCAGCGTGTCTA	GGAAAAAGTAGGAGCCCCAC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.288783	123556	124305	1332-1342	0.666667	67.3