Hotspot

ycf1

SSC · 124737-130292 · 5556 bp

Back to Gelsemium

Pi 0.0162

MarkerSeek score 51.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5556
variable_sites: 90
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGTTTGGATTTTCGTGAGCG	TCAGACCAAGAACAAGGAAA	50.0	40.0	58.4	54.4	245 bp	1.0	52.2
ycf1_p2	CGTTTGGATTTTCGTGAGCG	TTCAGACCAAGAACAAGGAA	50.0	40.0	58.4	54.4	246 bp	1.0	52.2
ycf1_p3	CGTTTGGATTTTCGTGAGCG	TTTCAGACCAAGAACAAGGA	50.0	40.0	58.4	54.4	247 bp	1.0	52.2
ycf1_p4	CGTTTGGATTTTCGTGAGCG	TCAGACCAAGAACAAGGAAAA	50.0	38.1	58.4	55.3	245 bp	1.0	51.1
ycf1_p5	CGTTTGGATTTTCGTGAGCG	TTCAGACCAAGAACAAGGAAA	50.0	38.1	58.4	55.3	246 bp	1.0	51.1