Hotspot

atpF

LSC · 12198-13442 · 1245 bp

Back to Gelsemium

Pi 0.00884

MarkerSeek score 55.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1245
variable_sites: 11
pi: 0.00884
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTTCGTCAGCTCGAATGGTT	GGGGGAAGTTATACAAAAAGAACTC	45.0	40.0	57.8	57.8	1399 bp	1.0	40.6
atpF_p2	TTTCGTCAGCTCGAATGGTT	GGGGGAAGTTATACAAAAAGAACT	45.0	37.5	57.8	56.8	1399 bp	1.0	40.6
atpF_p3	TCGTCAGCTCGAATGGTTAC	GGGGGAAGTTATACAAAAAGAACTC	50.0	40.0	57.7	57.8	1397 bp	1.0	40.6
atpF_p4	TCGTCAGCTCGAATGGTTAC	GGGGGAAGTTATACAAAAAGAACT	50.0	37.5	57.7	56.8	1397 bp	1.0	40.6
atpF_p5	GTCAGCTCGAATGGTTACCA	GGGGGAAGTTATACAAAAAGAACTC	50.0	40.0	57.6	57.8	1395 bp	1.0	40.6