Hotspot

ndhG-ndhI

SSC · 119791-120011 · 221 bp

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Pi 0.0452

MarkerSeek score 66.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 221
variable_sites: 10
pi: 0.0452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GACCTACCCCCAGAAAGACT	CCCTTGATTCAAAAGAATTCCCA	55.0	39.1	58.0	57.3	311 bp	1.0	52.3
ndhG-ndhI_p2	AGACCTACCCCCAGAAAGAC	CCCTTGATTCAAAAGAATTCCCA	55.0	39.1	58.0	57.3	312 bp	1.0	52.2
ndhG-ndhI_p3	CAGACCTACCCCCAGAAAGA	CCCTTGATTCAAAAGAATTCCCA	55.0	39.1	58.0	57.3	313 bp	1.0	52.2
ndhG-ndhI_p4	TCAGACCTACCCCCAGAAAG	CCCTTGATTCAAAAGAATTCCCA	55.0	39.1	58.0	57.3	314 bp	1.0	52.2
ndhG-ndhI_p5	CCACCCCTAGACCTCCTAAG	CCCTTGATTCAAAAGAATTCCCA	60.0	39.1	57.9	57.3	335 bp	1.0	51.7