Hotspot

rpl36-infA

LSC · 81065-81124 · 60 bp

Back to Gelsemium

Pi 0.05

MarkerSeek score 70.00

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 3
pi: 0.05
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	TCACAAATTTTACGAACAGAAGCT	TTATCGACTTCGCAACAGGG	33.3	50.0	57.6	58.0	129 bp	1.0	46.8
rpl36-infA_p2	TCACAAATTTTACGAACAGAAGCT	GCAACAGGGATTCGAAGGAT	33.3	50.0	57.6	58.0	118 bp	1.0	46.7
rpl36-infA_p3	ACGAACAGAAGCTCTTATTTTCA	TTATCGACTTCGCAACAGGG	34.8	50.0	56.5	58.0	118 bp	1.0	45.9
rpl36-infA_p4	ACGAACAGAAGCTCTTATTTTCA	GCAACAGGGATTCGAAGGAT	34.8	50.0	56.5	58.0	107 bp	1.0	45.8
rpl36-infA_p5	ACAAATTTTACGAACAGAAGCTCT	TTATCGACTTCGCAACAGGG	33.3	50.0	57.4	58.0	127 bp	1.0	44.9