Hotspot

petB-petD

LSC · 77792-77979 · 188 bp

Back to Gelsemium

Pi 0.0286

MarkerSeek score 73.00

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 5
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	AACAGGGTATTTCGGGTCCT	TCCAATGCCAATGTGGTAGG	50.0	50.0	58.3	57.9	281 bp	1.0	81.3
petB-petD_p2	ACAGGGTATTTCGGGTCCTT	TCCAATGCCAATGTGGTAGG	50.0	50.0	58.3	57.9	280 bp	1.0	81.3
petB-petD_p3	AACAGGGTATTTCGGGTCCT	GTTCAAGTCACACACTCCCA	50.0	50.0	58.3	57.7	235 bp	1.0	80.4
petB-petD_p4	ACAGGGTATTTCGGGTCCTT	GTTCAAGTCACACACTCCCA	50.0	50.0	58.3	57.7	234 bp	1.0	80.4
petB-petD_p5	AAACAGGGTATTTCGGGTCC	TCCAATGCCAATGTGGTAGG	50.0	50.0	57.5	57.9	282 bp	1.0	80.0