Hotspot

atpB-rbcL

LSC · 56040-56763 · 724 bp

Back to Gelsemium

Pi 0.0097

MarkerSeek score 63.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 7
pi: 0.0097
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	831-833 bp	1.0	72.0
atpB-rbcL_p2	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	830-832 bp	1.0	71.8
atpB-rbcL_p3	ATGATTTGGACGATACGCCC	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	872-874 bp	1.0	71.7
atpB-rbcL_p4	GGAAACCCCAGAACCAGAAG	GTGGTGACATAAATCCCTCCC	55.0	52.4	58.1	58.1	792-794 bp	1.0	71.7
atpB-rbcL_p5	ATGATTTGGACGATACGCCC	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.1	58.1	871-873 bp	1.0	71.5