Hotspot

trnM-CAU-atpE

LSC · 53952-54156 · 205 bp

Back to Gelsemium

Pi 0.0537

MarkerSeek score 67.50

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 205
variable_sites: 11
pi: 0.0537
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GCTCCATGTGCTCTGATTCA	AGAGGCTATCAGTGGGGTTT	50.0	50.0	58.0	58.0	960-965 bp	1.0	81.0
trnM-CAU-atpE_p2	TCTATTTGCGCGGTATTGCT	TACGCCTTAACGACCAATGG	45.0	50.0	58.1	58.0	632-637 bp	1.0	81.0
trnM-CAU-atpE_p3	TGCGCGGTATTGCTTAGATT	TACGCCTTAACGACCAATGG	45.0	50.0	58.1	58.0	626-631 bp	1.0	81.0
trnM-CAU-atpE_p4	TTGCGCGGTATTGCTTAGAT	TACGCCTTAACGACCAATGG	45.0	50.0	58.1	58.0	627-632 bp	1.0	81.0
trnM-CAU-atpE_p5	GCTCCATGTGCTCTGATTCA	TACGCCTTAACGACCAATGG	50.0	50.0	58.0	58.0	1186-1191 bp	1.0	80.8