Hotspot

petN-psbM

LSC · 29550-30730 · 1181 bp

Back to Gelsemium

Pi 0.109
MarkerSeek score 84.40
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1181
variable_sites
124
pi
0.109
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTCGTAGTGTGGGGAAGAA TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.0 58.0 1263-1277 bp 1.0 81.9
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.0 58.0 1261-1275 bp 1.0 81.9
petN-psbM_p3 ACTCGTAGTGTGGGGAAGAA TCTAGTTCCTACCGCCTTTCT 50.0 47.6 58.0 57.9 1259-1273 bp 1.0 81.4
petN-psbM_p4 TCGTAGTGTGGGGAAGAAGT TCTAGTTCCTACCGCCTTTCT 50.0 47.6 58.0 57.9 1257-1271 bp 1.0 81.4
petN-psbM_p5 CGTAGTGTGGGGAAGAAGTG TCATTCTAGTTCCTACCGCCT 55.0 47.6 57.9 58.0 1260-1274 bp 1.0 81.3