Hotspot

trnH-GTG-psbA

LSC · 74-532 · 459 bp

Back to Gelsemium

Pi 0.103
MarkerSeek score 82.70
Primer available yes
Label trnH-GTG-psbA

Feature Metadata

label_name
trnH-GTG-psbA
feature_type
igs
region
LSC
length_bp
459
variable_sites
41
pi
0.103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GTG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 496-528 bp 1.0 88.8
trnH-GTG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 510-542 bp 1.0 88.6
trnH-GTG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 509-541 bp 1.0 88.6
trnH-GTG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 511-543 bp 1.0 88.5
trnH-GTG-psbA_p5 CGCATGGTGGATTCACAATC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.5 57.9 525-557 bp 1.0 85.6