primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	trnH-GTG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	74	532	496-528	1.000000	88.8
trnH-GTG-psbA_p2	trnH-GTG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	74	532	510-542	1.000000	88.6
trnH-GTG-psbA_p3	trnH-GTG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	74	532	509-541	1.000000	88.6
trnH-GTG-psbA_p4	trnH-GTG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	74	532	511-543	1.000000	88.5
trnH-GTG-psbA_p5	trnH-GTG-psbA	5	CGCATGGTGGATTCACAATC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.5	57.9	6.4	0.0	0.554952	74	532	525-557	1.000000	85.6
atpF_p1	atpF	1	TTTCGTCAGCTCGAATGGTT	GGGGGAAGTTATACAAAAAGAACTC	20	25	45.0	40.0	57.8	57.8	10.6	0.0	5.318253	12198	13442	1399-1399	1.000000	40.6
atpF_p2	atpF	2	TTTCGTCAGCTCGAATGGTT	GGGGGAAGTTATACAAAAAGAACT	20	24	45.0	37.5	57.8	56.8	10.6	0.0	5.322343	12198	13442	1399-1399	1.000000	40.6
atpF_p3	atpF	3	TCGTCAGCTCGAATGGTTAC	GGGGGAAGTTATACAAAAAGAACTC	20	25	50.0	40.0	57.7	57.8	0.0	0.0	5.427805	12198	13442	1397-1397	1.000000	40.6
atpF_p4	atpF	4	TCGTCAGCTCGAATGGTTAC	GGGGGAAGTTATACAAAAAGAACT	20	24	50.0	37.5	57.7	56.8	0.0	0.0	5.431894	12198	13442	1397-1397	1.000000	40.6
atpF_p5	atpF	5	GTCAGCTCGAATGGTTACCA	GGGGGAAGTTATACAAAAAGAACTC	20	25	50.0	40.0	57.6	57.8	6.5	0.0	5.534519	12198	13442	1395-1395	1.000000	40.6
atpF-atpH_p1	atpF-atpH	1	TATTAACCCCGAAACTCCCG	AGGCAGAGGGAAAAATACGG	20	20	50.0	50.0	57.0	57.6	0.0	0.0	1.406016	13443	13794	538-539	1.000000	71.3
atpF-atpH_p2	atpF-atpH	2	GGTATTAACCCCGAAACTCCC	AGGCAGAGGGAAAAATACGG	21	20	52.4	50.0	58.1	57.6	0.0	0.0	1.496938	13443	13794	540-541	1.000000	70.5
atpF-atpH_p3	atpF-atpH	3	TATTAACCCCGAAACTCCCG	GCAGAGGGAAAAATACGGGG	20	20	50.0	55.0	57.0	58.6	0.0	0.0	1.596402	13443	13794	536-537	1.000000	69.8
atpF-atpH_p4	atpF-atpH	4	TATTAACCCCGAAACTCCCG	GGCAGAGGGAAAAATACGGG	20	20	50.0	55.0	57.0	58.6	0.0	0.0	1.596402	13443	13794	537-538	1.000000	69.8
atpF-atpH_p5	atpF-atpH	5	GGTATTAACCCCGAAACTCCC	GCAGAGGGAAAAATACGGGG	21	20	52.4	55.0	58.1	58.6	0.0	0.0	1.687324	13443	13794	538-539	1.000000	69.0
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCCT	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.033465	29550	30730	1263-1277	1.000000	81.9
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCCT	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.033465	29550	30730	1261-1275	1.000000	81.9
petN-psbM_p3	petN-psbM	3	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACCGCCTTTCT	20	21	50.0	47.6	58.0	57.9	0.0	0.0	1.102712	29550	30730	1259-1273	1.000000	81.4
petN-psbM_p4	petN-psbM	4	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACCGCCTTTCT	20	21	50.0	47.6	58.0	57.9	0.0	0.0	1.102712	29550	30730	1257-1271	1.000000	81.4
petN-psbM_p5	petN-psbM	5	CGTAGTGTGGGGAAGAAGTG	TCATTCTAGTTCCTACCGCCT	20	21	55.0	47.6	57.9	58.0	0.0	0.0	1.108380	29550	30730	1260-1274	1.000000	81.3
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCATATTCGTAAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	22	20	31.8	50.0	53.9	57.8	0.0	0.0	6.319809	51869	53028	1216-1225	1.000000	43.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	AGCCAAAATAGGAATAAGACTTGA	AGAAGGTCTACGGTTCGAGT	24	20	33.3	50.0	56.1	57.8	0.0	0.0	6.111659	51869	53028	1265-1274	1.000000	42.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	AAGCCAAAATAGGAATAAGACTTGA	AGAAGGTCTACGGTTCGAGT	25	20	32.0	50.0	56.7	57.8	0.0	0.0	6.453976	51869	53028	1266-1275	1.000000	42.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	AGCCAAAATAGGAATAAGACTTGA	GAAGGTCTACGGTTCGAGTC	24	20	33.3	55.0	56.1	57.5	0.0	0.0	6.455431	51869	53028	1264-1273	1.000000	42.9
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GCCAAAATAGGAATAAGACTTGA	AGAAGGTCTACGGTTCGAGT	23	20	34.8	50.0	54.7	57.8	0.0	0.0	6.511347	51869	53028	1264-1273	1.000000	42.9
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	GCTCCATGTGCTCTGATTCA	AGAGGCTATCAGTGGGGTTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061517	53952	54156	960-965	1.000000	81.0
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	TCTATTTGCGCGGTATTGCT	TACGCCTTAACGACCAATGG	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.064833	53952	54156	632-637	1.000000	81.0
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	TGCGCGGTATTGCTTAGATT	TACGCCTTAACGACCAATGG	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.064833	53952	54156	626-631	1.000000	81.0
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	TTGCGCGGTATTGCTTAGAT	TACGCCTTAACGACCAATGG	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.064833	53952	54156	627-632	1.000000	81.0
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	GCTCCATGTGCTCTGATTCA	TACGCCTTAACGACCAATGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047041	53952	54156	1186-1191	1.000000	80.8
atpB-rbcL_p1	atpB-rbcL	1	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	58.1	58.0	0.0	0.0	1.106208	56040	56763	831-833	1.000000	72.0
atpB-rbcL_p2	atpB-rbcL	2	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	58.1	58.1	0.0	0.0	1.139495	56040	56763	830-832	1.000000	71.8
atpB-rbcL_p3	atpB-rbcL	3	ATGATTTGGACGATACGCCC	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.1	58.0	0.0	0.0	1.138312	56040	56763	872-874	1.000000	71.7
atpB-rbcL_p4	atpB-rbcL	4	GGAAACCCCAGAACCAGAAG	GTGGTGACATAAATCCCTCCC	20	21	55.0	52.4	58.1	58.1	0.0	0.0	1.156682	56040	56763	792-794	1.000000	71.7
atpB-rbcL_p5	atpB-rbcL	5	ATGATTTGGACGATACGCCC	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	58.1	58.1	0.0	0.0	1.171600	56040	56763	871-873	1.000000	71.5
psbL-psbF_p1	psbL-psbF	1	TCGTTCGGGTTTGATTGTGT	AGTACGATGGTTGGCTGTTC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.138465	65866	65888	134-134	1.000000	80.1
psbL-psbF_p2	psbL-psbF	2	TCGTTCGGGTTTGATTGTGT	CAGTACGATGGTTGGCTGTT	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.235293	65866	65888	135-135	1.000000	79.3
psbL-psbF_p3	psbL-psbF	3	TCGTTCGGGTTTGATTGTGT	GTACGATGGTTGGCTGTTCA	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.240369	65866	65888	133-133	1.000000	79.3
psbL-psbF_p4	psbL-psbF	4	TCGTTCGGGTTTGATTGTGT	TACGATGGTTGGCTGTTCAC	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.240369	65866	65888	132-132	1.000000	79.3
psbL-psbF_p5	psbL-psbF	5	TCGTTCGGGTTTGATTGTGT	CCTAGCTGTACCTACCGTCT	20	20	45.0	55.0	58.0	57.7	0.0	0.0	0.388828	65866	65888	110-110	1.000000	78.3
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGCGGAGGTTATAGTTAAAGCC	20	22	50.0	45.5	57.9	57.9	6.4	0.0	2.120635	66270	67283	1178-1185	1.000000	69.8
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AGCGGAGGTTATAGTTAAAGCC	20	22	50.0	45.5	57.8	57.9	24.9	0.0	2.225643	66270	67283	1101-1108	1.000000	69.4
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AGCGGAGGTTATAGTTAAAGCC	20	22	45.0	45.5	58.2	57.9	0.0	0.0	2.240321	66270	67283	1187-1194	1.000000	68.8
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	GCGGAGGTTATAGTTAAAGCCA	20	22	50.0	45.5	57.9	58.2	6.4	0.0	2.250803	66270	67283	1177-1184	1.000000	68.8
psbE-petL_p5	psbE-petL	5	TGCTGACGAATAACCAACCC	AGCGGAGGTTATAGTTAAAGCC	20	22	50.0	45.5	58.2	57.9	0.0	0.0	2.253226	66270	67283	1192-1199	1.000000	68.7
petB-petD_p1	petB-petD	1	AACAGGGTATTTCGGGTCCT	TCCAATGCCAATGTGGTAGG	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.477219	77792	77979	281-281	1.000000	81.3
petB-petD_p2	petB-petD	2	ACAGGGTATTTCGGGTCCTT	TCCAATGCCAATGTGGTAGG	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.477219	77792	77979	280-280	1.000000	81.3
petB-petD_p3	petB-petD	3	AACAGGGTATTTCGGGTCCT	GTTCAAGTCACACACTCCCA	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.660446	77792	77979	235-235	1.000000	80.4
petB-petD_p4	petB-petD	4	ACAGGGTATTTCGGGTCCTT	GTTCAAGTCACACACTCCCA	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.660446	77792	77979	234-234	1.000000	80.4
petB-petD_p5	petB-petD	5	AAACAGGGTATTTCGGGTCC	TCCAATGCCAATGTGGTAGG	20	20	50.0	50.0	57.5	57.9	0.0	0.0	0.644499	77792	77979	282-282	1.000000	80.0
petD-rps11_p1	petD-rps11	1	TTGGTTGGGTATTGGTGCAA	ACTCCGGGCTATTCGTAGAA	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.151075	79182	80446	1429-1433	1.000000	79.2
petD-rps11_p2	petD-rps11	2	TTGGTTGGGTATTGGTGCAA	TCCGGGCTATTCGTAGAAGT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.151075	79182	80446	1427-1431	1.000000	79.2
petD-rps11_p3	petD-rps11	3	TTTGGTTGGGTATTGGTGCA	ACTCCGGGCTATTCGTAGAA	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.151075	79182	80446	1430-1434	1.000000	79.2
petD-rps11_p4	petD-rps11	4	TTTGGTTGGGTATTGGTGCA	TCCGGGCTATTCGTAGAAGT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.151075	79182	80446	1428-1432	1.000000	79.2
petD-rps11_p5	petD-rps11	5	CGTCGTCCTGTAGCTACAAC	TCCGGGCTATTCGTAGAAGT	20	20	55.0	50.0	58.1	57.9	25.9	0.0	0.170503	79182	80446	1477-1481	1.000000	79.1
rpl36-infA_p1	rpl36-infA	1	TCACAAATTTTACGAACAGAAGCT	TTATCGACTTCGCAACAGGG	24	20	33.3	50.0	57.6	58.0	0.0	0.0	4.376392	81065	81124	129-129	1.000000	46.8
rpl36-infA_p2	rpl36-infA	2	TCACAAATTTTACGAACAGAAGCT	GCAACAGGGATTCGAAGGAT	24	20	33.3	50.0	57.6	58.0	0.0	3.3	4.415932	81065	81124	118-118	1.000000	46.7
rpl36-infA_p3	rpl36-infA	3	ACGAACAGAAGCTCTTATTTTCA	TTATCGACTTCGCAACAGGG	23	20	34.8	50.0	56.5	58.0	0.0	0.0	4.519141	81065	81124	118-118	1.000000	45.9
rpl36-infA_p4	rpl36-infA	4	ACGAACAGAAGCTCTTATTTTCA	GCAACAGGGATTCGAAGGAT	23	20	34.8	50.0	56.5	58.0	0.0	3.3	4.558682	81065	81124	107-107	1.000000	45.8
rpl36-infA_p5	rpl36-infA	5	ACAAATTTTACGAACAGAAGCTCT	TTATCGACTTCGCAACAGGG	24	20	33.3	50.0	57.4	58.0	0.0	0.0	4.624383	81065	81124	127-127	1.000000	44.9
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	109215	109786	713-713	1.000000	78.7
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	109215	109786	664-664	1.000000	77.5
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.483095	109215	109786	668-668	1.000000	76.6
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	TCTCCCGTTGTTCCTCAGTA	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.513605	109215	109786	717-717	1.000000	76.3
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.526686	109215	109786	707-707	1.000000	76.2
ndhF-rpl32_p1	ndhF-rpl32	1	TGCCGTGGGAAAAAGAAGAA	CGCTGTCCAATATCCCTTCC	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.175815	113477	114243	946-947	1.000000	80.4
ndhF-rpl32_p2	ndhF-rpl32	2	TTGCCGTGGGAAAAAGAAGA	CGCTGTCCAATATCCCTTCC	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.175815	113477	114243	947-948	1.000000	80.4
ndhF-rpl32_p3	ndhF-rpl32	3	GTTGCCGTGGGAAAAAGAAG	CGCTGTCCAATATCCCTTCC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.249966	113477	114243	948-949	1.000000	79.8
ndhF-rpl32_p4	ndhF-rpl32	4	TTTGTTGCCGTGGGAAAAAG	CGCTGTCCAATATCCCTTCC	20	20	45.0	55.0	57.7	58.1	0.0	0.0	0.417342	113477	114243	951-952	1.000000	78.4
ndhF-rpl32_p5	ndhF-rpl32	5	TGTTGCCGTGGGAAAAAGAA	CGCTGTCCAATATCCCTTCC	20	20	45.0	55.0	58.5	58.1	0.0	0.0	0.640362	113477	114243	949-950	1.000000	76.6
ndhG-ndhI_p1	ndhG-ndhI	1	GACCTACCCCCAGAAAGACT	CCCTTGATTCAAAAGAATTCCCA	20	23	55.0	39.1	58.0	57.3	0.0	0.6	3.789601	119791	120011	311-311	1.000000	52.3
ndhG-ndhI_p2	ndhG-ndhI	2	AGACCTACCCCCAGAAAGAC	CCCTTGATTCAAAAGAATTCCCA	20	23	55.0	39.1	58.0	57.3	0.0	0.6	3.789601	119791	120011	312-312	1.000000	52.2
ndhG-ndhI_p3	ndhG-ndhI	3	CAGACCTACCCCCAGAAAGA	CCCTTGATTCAAAAGAATTCCCA	20	23	55.0	39.1	58.0	57.3	0.0	0.6	3.791015	119791	120011	313-313	1.000000	52.2
ndhG-ndhI_p4	ndhG-ndhI	4	TCAGACCTACCCCCAGAAAG	CCCTTGATTCAAAAGAATTCCCA	20	23	55.0	39.1	58.0	57.3	0.0	0.6	3.791015	119791	120011	314-314	1.000000	52.2
ndhG-ndhI_p5	ndhG-ndhI	5	CCACCCCTAGACCTCCTAAG	CCCTTGATTCAAAAGAATTCCCA	20	23	60.0	39.1	57.9	57.3	0.0	0.6	3.833237	119791	120011	335-335	1.000000	51.7
ndhI-ndhA_p1	ndhI-ndhA	1	AACCGATTCGTGTGAGACAG	CAAGGCTGAGAATGGACCAA	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.271015	120552	120649	312-312	1.000000	78.6
ndhI-ndhA_p2	ndhI-ndhA	2	AACCGATTCGTGTGAGACAG	AAGGCTGAGAATGGACCAAC	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.272569	120552	120649	311-311	1.000000	78.6
ndhI-ndhA_p3	ndhI-ndhA	3	TGGCAGCTCGTATTGTTTGT	CAAGGCTGAGAATGGACCAA	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.314146	120552	120649	263-263	1.000000	78.4
ndhI-ndhA_p4	ndhI-ndhA	4	TGGCAGCTCGTATTGTTTGT	AAGGCTGAGAATGGACCAAC	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.315700	120552	120649	262-262	1.000000	78.4
ndhI-ndhA_p5	ndhI-ndhA	5	TGAAACCTTGGCCGATGTAC	CAAGGCTGAGAATGGACCAA	20	20	50.0	50.0	58.2	57.8	0.7	0.0	0.388948	120552	120649	284-284	1.000000	77.7
ycf1_p1	ycf1	1	CGTTTGGATTTTCGTGAGCG	TCAGACCAAGAACAAGGAAA	20	20	50.0	40.0	58.4	54.4	0.0	0.0	4.003319	128434	128553	245-245	1.000000	52.2
ycf1_p2	ycf1	2	CGTTTGGATTTTCGTGAGCG	TTCAGACCAAGAACAAGGAA	20	20	50.0	40.0	58.4	54.4	0.0	0.0	4.003319	128434	128553	246-246	1.000000	52.2
ycf1_p3	ycf1	3	CGTTTGGATTTTCGTGAGCG	TTTCAGACCAAGAACAAGGA	20	20	50.0	40.0	58.4	54.4	0.0	0.0	4.003319	128434	128553	247-247	1.000000	52.2
ycf1_p4	ycf1	4	CGTTTGGATTTTCGTGAGCG	TCAGACCAAGAACAAGGAAAA	20	21	50.0	38.1	58.4	55.3	0.0	0.0	4.146839	128434	128553	245-245	1.000000	51.1
ycf1_p5	ycf1	5	CGTTTGGATTTTCGTGAGCG	TTCAGACCAAGAACAAGGAAA	20	21	50.0	38.1	58.4	55.3	0.0	0.0	4.146839	128434	128553	246-246	1.000000	51.1