Hotspot

ycf3

LSC · 44396-46395 · 2000 bp

Back to Gelidocalamus

Pi 0.000985

MarkerSeek score 54.80

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2000
variable_sites: 13
pi: 0.000985
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ATCAACAACCAAACCACCCA	AGAGCATATGGATCGATCGA	45.0	45.0	57.8	55.7	2127-2144 bp	1.0	61.8
ycf3_p2	TCAACAACCAAACCACCCAT	AGAGCATATGGATCGATCGA	45.0	45.0	57.8	55.7	2126-2143 bp	1.0	61.8
ycf3_p3	ATCAACAACCAAACCACCCA	AGAGCATATGGATCGATCGAT	45.0	42.9	57.8	56.3	2127-2144 bp	1.0	58.3
ycf3_p4	TCAACAACCAAACCACCCAT	AGAGCATATGGATCGATCGAT	45.0	42.9	57.8	56.3	2126-2143 bp	1.0	58.3
ycf3_p5	GATCAACAACCAAACCACCC	AGAGCATATGGATCGATCGA	50.0	45.0	57.2	55.7	2128-2145 bp	1.0	56.7