Hotspot

infA

LSC · 78810-79133 · 324 bp

Back to Gelidocalamus

Pi 0.00231

MarkerSeek score 26.90

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 324
variable_sites: 2
pi: 0.00231
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	ATACGCACTCTCCTACGTGA	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.0	58.1	1051-1088 bp	1.0	81.0
infA_p2	TCCACTACCAGCACCCTTTA	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.0	58.1	1322-1359 bp	1.0	80.7
infA_p3	TTGCAAACCCACTGTACGAA	TTAAAGCGTATCAGCCGACC	45.0	50.0	58.0	58.1	1286-1323 bp	1.0	80.7
infA_p4	GTAATGCGGCATCTCTTCCA	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.0	58.1	1338-1375 bp	1.0	80.6
infA_p5	TAATGCGGCATCTCTTCCAC	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.0	58.1	1337-1374 bp	1.0	80.6