Hotspot

ndhG-ndhI

SSC · 113831-114076 · 246 bp

Back to Gelidocalamus

Pi 0.0115

MarkerSeek score 51.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 246
variable_sites: 14
pi: 0.0115
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAATCTCCA	50.0	50.0	58.2	56.7	483-490 bp	1.0	69.9
ndhG-ndhI_p2	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAATCTCC	50.0	50.0	58.2	56.7	484-491 bp	1.0	69.9
ndhG-ndhI_p3	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAATCTCCA	50.0	47.6	58.2	58.5	484-491 bp	1.0	68.9
ndhG-ndhI_p4	CCCAGCGAAAAGGCAGAATA	TTGAGTCGGTTACCAATCTCC	50.0	47.6	58.2	57.4	485-492 bp	1.0	67.8
ndhG-ndhI_p5	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAATCTCCAT	50.0	47.6	58.2	57.2	483-490 bp	1.0	66.1