Hotspot

infA-rps8

LSC · 79134-79244 · 111 bp

Back to Gelidocalamus

Pi 0.0388

MarkerSeek score 52.40

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 111
variable_sites: 13
pi: 0.0388
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	TGGTGTCATTCTCTAGGCGA	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.2	58.1	406-407 bp	1.0	81.6
infA-rps8_p2	ATAGCCCAGGATGATGGTGT	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.2	58.1	420-421 bp	1.0	81.3
infA-rps8_p3	TTGACCCTATCCCCCATCAG	TTAAAGCGTATCAGCCGACC	55.0	50.0	58.2	58.1	476-477 bp	1.0	80.9
infA-rps8_p4	TGACTTTTGCTTCTCTCGGG	TTAAAGCGTATCAGCCGACC	50.0	50.0	57.8	58.1	337-338 bp	0.882353	77.2
infA-rps8_p5	GACTTTTGCTTCTCTCGGGT	TTAAAGCGTATCAGCCGACC	50.0	50.0	57.8	58.1	336-337 bp	0.882353	77.2