Hotspot

trnG-UCC-trnT-GGU

LSC · 14323-16308 · 1986 bp

Back to Gelidocalamus

Pi 0.00537

MarkerSeek score 60.40

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1986
variable_sites: 39
pi: 0.00537
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	38.1	52.4	55.5	58.1	1854-2144 bp	1.0	48.0
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	GACTTACGTCTTACCATGGCA	38.1	47.6	55.5	57.8	1867-2157 bp	1.0	47.3
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	TGACTTACGTCTTACCATGGC	38.1	47.6	55.5	57.8	1868-2158 bp	1.0	47.3
trnG-UCC-trnT-GGU_p4	AGAACGAATCACACTTTTACCAC	CCATGGCATTACTCTACCACC	39.1	52.4	57.3	58.1	1857-2147 bp	1.0	46.9
trnG-UCC-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	39.1	52.4	57.3	58.1	1856-2146 bp	1.0	46.9