Hotspot

rps16-trnQ-UUG

LSC · 5607-7139 · 1533 bp

Back to Gelidocalamus

Pi 0.00283

MarkerSeek score 50.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1533
variable_sites: 22
pi: 0.00283
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1603-1638 bp	1.0	80.1
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1614-1649 bp	1.0	79.5
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1607-1642 bp	1.0	79.0
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	1618-1653 bp	1.0	78.5
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TAAAACCAGAAATGGGGCGT	45.0	45.0	58.1	57.7	1663-1698 bp	1.0	78.3