Hotspot

ORF256

Genome · 51649-52407 · 759 bp

Back to Gelidium

Pi 0.0523

MarkerSeek score 79.20

Primer available yes

Label ORF256

Feature Metadata

label_name: ORF256
feature_type: gene
region: Genome
length_bp: 759
variable_sites: 71
pi: 0.0523
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF256_p1	GGGCAATGAGTATCTGGTGG	TGCGTATACCAATTTCGCCA	55.0	45.0	58.0	58.0	1563-1565 bp	0.75	73.3
ORF256_p2	GGGCAATGAGTATCTGGTGG	ATCTGAGGGCCTAGCAGTAG	55.0	55.0	58.0	58.0	1868-1870 bp	0.75	73.1
ORF256_p3	GGGCAATGAGTATCTGGTGG	ACCAATTTCGCCATCTACCC	55.0	50.0	58.0	57.9	1556-1558 bp	0.75	73.1
ORF256_p4	GGGCAATGAGTATCTGGTGG	CCAATTTCGCCATCTACCCT	55.0	50.0	58.0	57.7	1555-1557 bp	0.75	70.7
ORF256_p5	GGGCAATGAGTATCTGGTGG	AAATCTGAGGGCCTAGCAGT	55.0	50.0	58.0	58.4	1870-1872 bp	0.75	69.9