Hotspot

trnV-trnY

Genome · 152524-152577 · 54 bp

Back to Gelidium

Pi 0.102

MarkerSeek score 73.60

Primer available yes

Label trnV-trnY

Feature Metadata

label_name: trnV-trnY
feature_type: igs
region: Genome
length_bp: 54
variable_sites: 10
pi: 0.102
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-trnY_p1	TCTGGCAAAGCAACAAGACT	AATGTGGGCAGCACAGTTTA	45.0	45.0	57.9	58.0	945-949 bp	1.0	84.1
trnV-trnY_p2	TCTGGCAAAGCAACAAGACT	TGTGGGCAGCACAGTTTATT	45.0	45.0	57.9	58.0	943-947 bp	1.0	84.1
trnV-trnY_p3	ACGCAGTAGATCTGGCAAAG	ACAGGTGATGCAAGCTTTCA	50.0	45.0	58.0	58.0	819-823 bp	0.75	72.8
trnV-trnY_p4	ACGCAGTAGATCTGGCAAAG	AATGTGGGCAGCACAGTTTA	50.0	45.0	58.0	58.0	955-959 bp	0.75	72.6
trnV-trnY_p5	ACGCAGTAGATCTGGCAAAG	TGTGGGCAGCACAGTTTATT	50.0	45.0	58.0	58.0	953-957 bp	0.75	72.6