Hotspot

dnaB-trnF

Genome · 149051-149096 · 46 bp

Back to Gelidium

Pi 0.13

MarkerSeek score 72.70

Primer available yes

Label dnaB-trnF

Feature Metadata

label_name: dnaB-trnF
feature_type: igs
region: Genome
length_bp: 46
variable_sites: 10
pi: 0.13
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB-trnF_p1	ATCGTAATGGACCAACTGGC	TCCACTGCTCTACCAACTGA	50.0	50.0	58.0	58.0	160 bp	1.0	86.1
dnaB-trnF_p2	ATCGTAATGGACCAACTGGC	TCAGTCCACTGCTCTACCAA	50.0	50.0	58.0	58.0	164 bp	1.0	86.0
dnaB-trnF_p3	ATCGTAATGGACCAACTGGC	AGTCCACTGCTCTACCAACT	50.0	50.0	58.0	58.0	162 bp	1.0	86.0
dnaB-trnF_p4	ATCGTAATGGACCAACTGGC	TTCAGTCCACTGCTCTACCA	50.0	50.0	58.0	58.0	165 bp	1.0	85.9
dnaB-trnF_p5	ATCGTAATGGACCAACTGGC	TGAGGAGGTGCTTTCTGGTA	50.0	50.0	58.0	58.0	376 bp	0.5	59.7