Hotspot

ycf27-psbD

Genome · 127635-128594 · 960 bp

Back to Gelidium

Pi 0.0918

MarkerSeek score 86.50

Primer available yes

Label ycf27-psbD

Feature Metadata

label_name: ycf27-psbD
feature_type: igs
region: Genome
length_bp: 960
variable_sites: 159
pi: 0.0918
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf27-psbD_p1	AACAAAGCCACAATTGCACC	CTGCCACACCAAAACTAGGT	45.0	50.0	58.0	58.0	1846-2583 bp	0.75	77.3
ycf27-psbD_p2	TTAGTGCCTCTGATGGGGAA	TTCAGGCCCCCACAGTAATA	50.0	50.0	58.0	58.0	1853-1877 bp	0.75	74.3
ycf27-psbD_p3	TTAGTGCCTCTGATGGGGAA	GCCCATAATCCGCCTATCTG	50.0	55.0	58.0	58.0	1900-1924 bp	0.75	74.2
ycf27-psbD_p4	TTAGTGCCTCTGATGGGGAA	AGGGCCTGAAAATGCGATAG	50.0	50.0	58.0	58.0	2012-2036 bp	0.75	73.9
ycf27-psbD_p5	TTAGTGCCTCTGATGGGGAA	CTGCCACACCAAAACTAGGT	50.0	50.0	58.0	58.0	2118 bp	0.5	59.6