Hotspot

rps6-rrf

Genome · 121465-121926 · 462 bp

Back to Gelidium

Pi 0.0819

MarkerSeek score 72.50

Primer available yes

Label rps6-rrf

Feature Metadata

label_name: rps6-rrf
feature_type: igs
region: Genome
length_bp: 462
variable_sites: 70
pi: 0.0819
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps6-rrf_p1	ACAAAGCGGTGGAAAGAACA	TTCGGTCCATATCCGGTGTA	45.0	50.0	58.2	57.9	1121-1147 bp	1.0	85.3
rps6-rrf_p2	ACAAAGCGGTGGAAAGAACA	ATCTCCCCCAAGAGTTCACA	45.0	50.0	58.2	58.0	1266-1292 bp	1.0	84.7
rps6-rrf_p3	ACAAAGCGGTGGAAAGAACA	TCTCCCCCAAGAGTTCACAT	45.0	50.0	58.2	58.0	1265-1291 bp	1.0	84.7
rps6-rrf_p4	ACAAAGCGGTGGAAAGAACA	CAAGTCGAGCAGAGACGAAA	45.0	50.0	58.2	57.9	1366-1392 bp	1.0	84.1
rps6-rrf_p5	ACAAAGCGGTGGAAAGAACA	GGCAGTACTGAGTGGAAAGG	45.0	55.0	58.2	57.9	1329-1355 bp	1.0	83.9