Hotspot

trnV-trnR

Genome · 96774-96796 · 23 bp

Back to Gelidium

Pi 0.0725

MarkerSeek score 73.50

Primer available yes

Label trnV-trnR

Feature Metadata

label_name: trnV-trnR
feature_type: igs
region: Genome
length_bp: 23
variable_sites: 3
pi: 0.0725
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-trnR_p1	CGGTTAGCTCAGTGGTAGAG	GGTCCCTGTCCTAATCCCTT	55.0	55.0	57.1	58.1	121-126 bp	1.0	77.5
trnV-trnR_p2	CGGTTAGCTCAGTGGTAGAG	AAGGTCCCTGTCCTAATCCC	55.0	55.0	57.1	58.1	123-128 bp	1.0	77.4
trnV-trnR_p3	CTGGTTCAAGTCCAGTACCG	AAGGTCCCTGTCCTAATCCC	55.0	55.0	57.9	58.1	82-83 bp	0.75	69.5
trnV-trnR_p4	CTGGTTCAAGTCCAGTACCG	GGTCCCTGTCCTAATCCCTT	55.0	55.0	57.9	58.1	80-81 bp	0.75	69.5
trnV-trnR_p5	CTGGTTCAAGTCCAGTACCG	AGGTCCCTGTCCTAATCCCT	55.0	55.0	57.9	59.0	81-82 bp	0.75	62.6