Hotspot

psaL-trnT

Genome · 60812-61289 · 478 bp

Back to Gelidium

Pi 0.148

MarkerSeek score 69.30

Primer available yes

Label psaL-trnT

Feature Metadata

label_name: psaL-trnT
feature_type: igs
region: Genome
length_bp: 478
variable_sites: 126
pi: 0.148
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaL-trnT_p1	GAAATTGGTATGGCGCATGG	TGTAATCAGCAGGTTGTGGG	50.0	50.0	57.9	57.8	619-879 bp	1.0	89.3
psaL-trnT_p2	GAAATTGGTATGGCGCATGG	GTAATCAGCAGGTTGTGGGT	50.0	50.0	57.9	57.8	618-878 bp	1.0	89.3
psaL-trnT_p3	TGGGGACAATTTACAGCTGG	TGTAATCAGCAGGTTGTGGG	50.0	50.0	57.8	57.8	418-678 bp	1.0	88.7
psaL-trnT_p4	TGGGGACAATTTACAGCTGG	GTAATCAGCAGGTTGTGGGT	50.0	50.0	57.8	57.8	417-677 bp	1.0	88.7
psaL-trnT_p5	GGGGACAATTTACAGCTGGT	TGTAATCAGCAGGTTGTGGG	50.0	50.0	57.8	57.8	417-677 bp	1.0	88.7