Hotspot

acsF-petN

Genome · 15099-15522 · 424 bp

Back to Gelidium

Pi 0.046

MarkerSeek score 72.20

Primer available yes

Label acsF-petN

Feature Metadata

label_name: acsF-petN
feature_type: igs
region: Genome
length_bp: 424
variable_sites: 36
pi: 0.046
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
acsF-petN_p1	TTCGGCCATTACAGGATTGG	ATCGGATTCCCCAGTACTGT	50.0	50.0	57.9	57.8	1185 bp	0.5	58.3
acsF-petN_p2	CCTTGCTTCATCTCGTGACA	ATCGGATTCCCCAGTACTGT	50.0	50.0	57.9	57.8	1218 bp	0.5	58.0
acsF-petN_p3	GCATTCGGCCATTACAGGAT	ATCGGATTCCCCAGTACTGT	50.0	50.0	58.4	57.8	1188 bp	0.5	55.6
acsF-petN_p4	AAGCATTCGGCCATTACAGG	ATCGGATTCCCCAGTACTGT	50.0	50.0	58.6	57.8	1190 bp	0.5	53.9
acsF-petN_p5	ATGCCTTGCTTCATCTCGTG	ATCGGATTCCCCAGTACTGT	50.0	50.0	58.6	57.8	1221 bp	0.5	53.7