primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-psaD_p1	trnS-psaD	1	ACCAACTCTACCACCTCTCC	AGCAAGAAAAGAGCAGTGCT	20	20	55.0	45.0	58.1	58.0	0.0	19.9	0.081566	11129	13431	343-2564	1.000000	91.3
trnS-psaD_p2	trnS-psaD	2	ACCAACTCTACCACCTCTCC	GGTAGTACAGGGGGATGGTT	20	20	55.0	55.0	58.1	58.1	0.0	2.1	0.170846	11129	13431	483-2704	1.000000	90.6
trnS-psaD_p3	trnS-psaD	3	ACCAACTCTACCACCTCTCC	CCGATAGAATCAGCAAGCGT	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.201929	11129	13431	719-2979	1.000000	90.4
trnS-psaD_p4	trnS-psaD	4	ATACCTTACGGAGAGGGTGG	AGCAAGAAAAGAGCAGTGCT	20	20	55.0	45.0	57.9	58.0	0.0	19.9	0.105174	11129	13431	1891-2638	0.750000	81.2
trnS-psaD_p5	trnS-psaD	5	ATACCTTACGGAGAGGGTGG	GGTAGTACAGGGGGATGGTT	20	20	55.0	55.0	57.9	58.1	0.0	2.1	0.194454	11129	13431	2031-2778	0.750000	80.4
acsF-petN_p1	acsF-petN	1	TTCGGCCATTACAGGATTGG	ATCGGATTCCCCAGTACTGT	20	20	50.0	50.0	57.9	57.8	2.2	21.2	0.227582	15099	15522	1185-1185	0.500000	58.3
acsF-petN_p2	acsF-petN	2	CCTTGCTTCATCTCGTGACA	ATCGGATTCCCCAGTACTGT	20	20	50.0	50.0	57.9	57.8	0.0	21.2	0.260857	15099	15522	1218-1218	0.500000	58.0
acsF-petN_p3	acsF-petN	3	GCATTCGGCCATTACAGGAT	ATCGGATTCCCCAGTACTGT	20	20	50.0	50.0	58.4	57.8	2.2	21.2	0.567040	15099	15522	1188-1188	0.500000	55.6
acsF-petN_p4	acsF-petN	4	AAGCATTCGGCCATTACAGG	ATCGGATTCCCCAGTACTGT	20	20	50.0	50.0	58.6	57.8	2.2	21.2	0.783658	15099	15522	1190-1190	0.500000	53.9
acsF-petN_p5	acsF-petN	5	ATGCCTTGCTTCATCTCGTG	ATCGGATTCCCCAGTACTGT	20	20	50.0	50.0	58.6	57.8	0.0	21.2	0.799705	15099	15522	1221-1221	0.500000	53.7
infB_p1	infB	1	CATGCCATCCCGGTAGTAAC	TATCACGTCGCTCTACTGGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.155151	30859	33060	2638-2638	1.000000	84.6
infB_p2	infB	2	CATGCCATCCCGGTAGTAAC	ACGTCGCTCTACTGGTCTTA	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.226053	30859	33060	2634-2634	1.000000	84.1
infB_p3	infB	3	CATGCCATCCCGGTAGTAAC	GGGAAAAACAAAGGAGCACG	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.255248	30859	33060	2949-2949	1.000000	83.6
infB_p4	infB	4	CATGCCATCCCGGTAGTAAC	TGGGGAAAAACAAAGGAGCA	20	20	55.0	45.0	58.1	57.8	0.0	0.0	0.287954	30859	33060	2951-2951	1.000000	83.3
infB_p5	infB	5	CATGCCATCCCGGTAGTAAC	GGAATACACCACAGCGCTTA	20	20	55.0	50.0	58.1	58.0	0.0	7.5	0.139589	30859	33060	2834-2834	0.500000	58.9
ORF256_p1	ORF256	1	GGGCAATGAGTATCTGGTGG	TGCGTATACCAATTTCGCCA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.052716	51649	52407	1563-1565	0.750000	73.3
ORF256_p2	ORF256	2	GGGCAATGAGTATCTGGTGG	ATCTGAGGGCCTAGCAGTAG	20	20	55.0	55.0	58.0	58.0	0.0	18.7	0.035370	51649	52407	1868-1870	0.750000	73.1
ORF256_p3	ORF256	3	GGGCAATGAGTATCTGGTGG	ACCAATTTCGCCATCTACCC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.079910	51649	52407	1556-1558	0.750000	73.1
ORF256_p4	ORF256	4	GGGCAATGAGTATCTGGTGG	CCAATTTCGCCATCTACCCT	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.375539	51649	52407	1555-1557	0.750000	70.7
ORF256_p5	ORF256	5	GGGCAATGAGTATCTGGTGG	AAATCTGAGGGCCTAGCAGT	20	20	55.0	50.0	58.0	58.4	0.0	18.7	0.437714	51649	52407	1870-1872	0.750000	69.9
ORF614_p1	ORF614	1	TGACCCATCAAGAAATCCTAACA	ACAAGGGCCGAATAGTAAGC	23	20	39.1	50.0	57.5	57.7	0.0	0.0	3.840142	54552	56471	2773-2788	0.750000	42.8
ORF614_p2	ORF614	2	TGACCCATCAAGAAATCCTAACA	CAAAACGTTGCAGTTCAGCA	23	20	39.1	45.0	57.5	58.1	0.0	0.0	3.606186	54552	56471	2861-2861	0.500000	31.2
ORF614_p3	ORF614	3	TGACCCATCAAGAAATCCTAACA	TCAAAACGTTGCAGTTCAGC	23	20	39.1	45.0	57.5	57.8	0.0	0.0	3.700293	54552	56471	2862-2862	0.500000	30.4
ORF614_p4	ORF614	4	TCAGGAACATAAGGATGCTTACA	CAAAACGTTGCAGTTCAGCA	23	20	39.1	45.0	57.3	58.1	9.0	0.0	3.785359	54552	56471	2952-2952	0.500000	29.7
ORF614_p5	ORF614	5	ACCCATCAAGAAATCCTAACAGA	CAAAACGTTGCAGTTCAGCA	23	20	39.1	45.0	57.2	58.1	0.0	0.0	3.872485	54552	56471	2859-2859	0.500000	29.0
ORF12_p1	ORF12	1	TATAGCATCTGTGGCCGAGA	CACGTGTGGGTCAACCTTTA	20	20	50.0	50.0	58.0	58.0	6.7	7.6	0.048327	58048	59241	2136-2139	0.750000	74.4
ORF12_p2	ORF12	2	TCTATAGCATCTGTGGCCGA	CACGTGTGGGTCAACCTTTA	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.048327	58048	59241	2138-2141	0.750000	74.4
ORF12_p3	ORF12	3	TATAGCATCTGTGGCCGAGA	ACTAACACGTGTGGGTCAAC	20	20	50.0	50.0	58.0	58.1	6.7	24.2	0.060592	58048	59241	2141-2144	0.750000	74.3
ORF12_p4	ORF12	4	TCTATAGCATCTGTGGCCGA	ACTAACACGTGTGGGTCAAC	20	20	50.0	50.0	58.0	58.1	0.0	24.2	0.060592	58048	59241	2143-2146	0.750000	74.3
ORF12_p5	ORF12	5	TATAGCATCTGTGGCCGAGA	CACTAACACGTGTGGGTCAA	20	20	50.0	50.0	58.0	58.1	6.7	24.2	0.061946	58048	59241	2142-2145	0.750000	74.3
psaL-trnT_p1	psaL-trnT	1	GAAATTGGTATGGCGCATGG	TGTAATCAGCAGGTTGTGGG	20	20	50.0	50.0	57.9	57.8	10.8	0.0	0.337423	60812	61289	619-879	1.000000	89.3
psaL-trnT_p2	psaL-trnT	2	GAAATTGGTATGGCGCATGG	GTAATCAGCAGGTTGTGGGT	20	20	50.0	50.0	57.9	57.8	10.8	0.0	0.338969	60812	61289	618-878	1.000000	89.3
psaL-trnT_p3	psaL-trnT	3	TGGGGACAATTTACAGCTGG	TGTAATCAGCAGGTTGTGGG	20	20	50.0	50.0	57.8	57.8	19.9	0.0	0.413203	60812	61289	418-678	1.000000	88.7
psaL-trnT_p4	psaL-trnT	4	TGGGGACAATTTACAGCTGG	GTAATCAGCAGGTTGTGGGT	20	20	50.0	50.0	57.8	57.8	19.9	0.0	0.414749	60812	61289	417-677	1.000000	88.7
psaL-trnT_p5	psaL-trnT	5	GGGGACAATTTACAGCTGGT	TGTAATCAGCAGGTTGTGGG	20	20	50.0	50.0	57.8	57.8	19.9	0.0	0.414769	60812	61289	417-677	1.000000	88.7
trnV-trnR_p1	trnV-trnR	1	CGGTTAGCTCAGTGGTAGAG	GGTCCCTGTCCTAATCCCTT	20	20	55.0	55.0	57.1	58.1	0.0	0.0	0.959032	96774	96796	121-126	1.000000	77.5
trnV-trnR_p2	trnV-trnR	2	CGGTTAGCTCAGTGGTAGAG	AAGGTCCCTGTCCTAATCCC	20	20	55.0	55.0	57.1	58.1	0.0	0.0	0.959032	96774	96796	123-128	1.000000	77.4
trnV-trnR_p3	trnV-trnR	3	CTGGTTCAAGTCCAGTACCG	AAGGTCCCTGTCCTAATCCC	20	20	55.0	55.0	57.9	58.1	4.1	0.0	0.185587	96774	96796	82-83	0.750000	69.5
trnV-trnR_p4	trnV-trnR	4	CTGGTTCAAGTCCAGTACCG	GGTCCCTGTCCTAATCCCTT	20	20	55.0	55.0	57.9	58.1	4.1	0.0	0.185587	96774	96796	80-81	0.750000	69.5
trnV-trnR_p5	trnV-trnR	5	CTGGTTCAAGTCCAGTACCG	AGGTCCCTGTCCTAATCCCT	20	20	55.0	55.0	57.9	59.0	4.1	0.0	1.052471	96774	96796	81-82	0.750000	62.6
ycf37_p1	ycf37	1	CGAGGCACCTGTATTTCTGG	TGTTGGTTGGGTTAGAAGGC	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.353641	100196	100648	1635-1636	1.000000	82.8
ycf37_p2	ycf37	2	AGAACCCTTTGGCCTTGTTT	TGTTGGTTGGGTTAGAAGGC	20	20	45.0	50.0	57.8	58.0	3.3	0.0	0.184979	100196	100648	1145-1145	0.750000	72.0
ycf37_p3	ycf37	3	AGAACCCTTTGGCCTTGTTT	TCGGGATATAGCGCAGTTTG	20	20	45.0	50.0	57.8	57.9	3.3	11.5	0.323601	100196	100648	744-744	0.750000	71.8
ycf37_p4	ycf37	4	GAACCCTTTGGCCTTGTTTG	TGTTGGTTGGGTTAGAAGGC	20	20	50.0	50.0	57.8	58.0	3.3	0.0	0.249236	100196	100648	1144-1144	0.500000	58.2
ycf37_p5	ycf37	5	GAACCCTTTGGCCTTGTTTG	TCGGGATATAGCGCAGTTTG	20	20	50.0	50.0	57.8	57.9	3.3	11.5	0.387858	100196	100648	743-743	0.500000	57.2
ccdA_p1	ccdA	1	AGCTGCGAACAACGATACAT	GGCGCAATTCTTCCAAACAA	20	20	45.0	45.0	58.0	57.9	0.0	10.8	0.157581	116775	117494	1878-1878	0.750000	71.9
ccdA_p2	ccdA	2	GGTTCAAATCCTTCCTGGGG	GGCGCAATTCTTCCAAACAA	20	20	55.0	45.0	58.1	57.9	0.0	10.8	0.294803	116775	117494	1309-1309	0.750000	70.9
ccdA_p3	ccdA	3	AGCTGCGAACAACGATACAT	CGAAAGAGTGCAGCCAGTTA	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.214377	116775	117494	1675-1675	0.500000	58.4
ccdA_p4	ccdA	4	CCACGTCCGCTTTAAAGGTA	GGCGCAATTCTTCCAAACAA	20	20	50.0	45.0	57.9	57.9	16.8	10.8	0.230602	116775	117494	1495-1495	0.500000	58.3
ccdA_p5	ccdA	5	CCACGTCCGCTTTAAAGGTA	CGAAAGAGTGCAGCCAGTTA	20	20	50.0	50.0	57.9	58.2	16.8	0.0	0.287399	116775	117494	1292-1292	0.500000	57.8
trpG-thiG_p1	trpG-thiG	1	AGATGGCCATTTTGTGTCCA	TGCCAAATACAGCTGGTTGT	20	20	45.0	45.0	57.7	58.0	21.8	19.9	0.313540	119408	119518	1236-1240	0.750000	71.1
trpG-thiG_p2	trpG-thiG	2	ACTTTTCCATGCACAGGCTT	GGTGTATTGTCTGGACGCAT	20	20	45.0	50.0	58.3	58.0	2.1	0.0	0.315834	119408	119518	520-524	0.750000	70.7
trpG-thiG_p3	trpG-thiG	3	ACTTTTCCATGCACAGGCTT	GTGAAGCAAGCAGAGCAATG	20	20	45.0	50.0	58.3	58.0	2.1	0.0	0.313117	119408	119518	624-628	0.750000	70.6
trpG-thiG_p4	trpG-thiG	4	ACTTTTCCATGCACAGGCTT	TGCCAAATACAGCTGGTTGT	20	20	45.0	45.0	58.3	58.0	2.1	19.9	0.297703	119408	119518	1008-1012	0.750000	70.3
trpG-thiG_p5	trpG-thiG	5	TTCCTGCTTTCTGTGGTGTG	TGCCAAATACAGCTGGTTGT	20	20	50.0	45.0	58.3	58.0	0.0	19.9	0.326905	119408	119518	878-882	0.750000	70.2
rps6-rrf_p1	rps6-rrf	1	ACAAAGCGGTGGAAAGAACA	TTCGGTCCATATCCGGTGTA	20	20	45.0	50.0	58.2	57.9	0.0	0.0	0.312623	121465	121926	1121-1147	1.000000	85.3
rps6-rrf_p2	rps6-rrf	2	ACAAAGCGGTGGAAAGAACA	ATCTCCCCCAAGAGTTCACA	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.287110	121465	121926	1266-1292	1.000000	84.7
rps6-rrf_p3	rps6-rrf	3	ACAAAGCGGTGGAAAGAACA	TCTCCCCCAAGAGTTCACAT	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.287110	121465	121926	1265-1291	1.000000	84.7
rps6-rrf_p4	rps6-rrf	4	ACAAAGCGGTGGAAAGAACA	CAAGTCGAGCAGAGACGAAA	20	20	45.0	50.0	58.2	57.9	0.0	0.0	0.294196	121465	121926	1366-1392	1.000000	84.1
rps6-rrf_p5	rps6-rrf	5	ACAAAGCGGTGGAAAGAACA	GGCAGTACTGAGTGGAAAGG	20	20	45.0	55.0	58.2	57.9	0.0	27.1	0.342922	121465	121926	1329-1355	1.000000	83.9
trnI-rrs_p1	trnI-rrs	1	TTTTCACCTTTCCCTCACGG	TAGTAATCGCCGGTCAGCTA	20	20	50.0	50.0	58.0	58.0	0.0	1.9	0.052859	125156	125221	968-968	1.000000	81.9
trnI-rrs_p2	trnI-rrs	2	TTTTCACCTTTCCCTCACGG	CCTTACGTTCTGGGCTACAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.030311	125156	125221	1104-1104	1.000000	81.8
trnI-rrs_p3	trnI-rrs	3	TTTTCACCTTTCCCTCACGG	CTAAGTTCGGATTGCAGGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054179	125156	125221	1017-1017	1.000000	81.8
trnI-rrs_p4	trnI-rrs	4	TTTTCACCTTTCCCTCACGG	CTAGATGTTGCGCGTATCGA	20	20	50.0	50.0	58.0	58.0	0.0	8.4	0.056754	125156	125221	1487-1487	1.000000	81.2
trnI-rrs_p5	trnI-rrs	5	TTTTCACCTTTCCCTCACGG	TCCGTGCAGTATCGTAGCTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.066672	125156	125221	1467-1467	1.000000	81.1
ycf27-psbD_p1	ycf27-psbD	1	AACAAAGCCACAATTGCACC	CTGCCACACCAAAACTAGGT	20	20	45.0	50.0	58.0	58.0	5.1	0.0	0.066551	127635	128594	1846-2583	0.750000	77.3
ycf27-psbD_p2	ycf27-psbD	2	TTAGTGCCTCTGATGGGGAA	TTCAGGCCCCCACAGTAATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.060558	127635	128594	1853-1877	0.750000	74.3
ycf27-psbD_p3	ycf27-psbD	3	TTAGTGCCTCTGATGGGGAA	GCCCATAATCCGCCTATCTG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.064424	127635	128594	1900-1924	0.750000	74.2
ycf27-psbD_p4	ycf27-psbD	4	TTAGTGCCTCTGATGGGGAA	AGGGCCTGAAAATGCGATAG	20	20	50.0	50.0	58.0	58.0	0.0	4.5	0.061714	127635	128594	2012-2036	0.750000	73.9
ycf27-psbD_p5	ycf27-psbD	5	TTAGTGCCTCTGATGGGGAA	CTGCCACACCAAAACTAGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.053329	127635	128594	2118-2118	0.500000	59.6
dnaB-trnF_p1	dnaB-trnF	1	ATCGTAATGGACCAACTGGC	TCCACTGCTCTACCAACTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048068	149051	149096	160-160	1.000000	86.1
dnaB-trnF_p2	dnaB-trnF	2	ATCGTAATGGACCAACTGGC	TCAGTCCACTGCTCTACCAA	20	20	50.0	50.0	58.0	58.0	0.0	1.5	0.048068	149051	149096	164-164	1.000000	86.0
dnaB-trnF_p3	dnaB-trnF	3	ATCGTAATGGACCAACTGGC	AGTCCACTGCTCTACCAACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.055292	149051	149096	162-162	1.000000	86.0
dnaB-trnF_p4	dnaB-trnF	4	ATCGTAATGGACCAACTGGC	TTCAGTCCACTGCTCTACCA	20	20	50.0	50.0	58.0	58.0	0.0	1.5	0.048068	149051	149096	165-165	1.000000	85.9
dnaB-trnF_p5	dnaB-trnF	5	ATCGTAATGGACCAACTGGC	TGAGGAGGTGCTTTCTGGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062563	149051	149096	376-376	0.500000	59.7
trnV-trnY_p1	trnV-trnY	1	TCTGGCAAAGCAACAAGACT	AATGTGGGCAGCACAGTTTA	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.063181	152524	152577	945-949	1.000000	84.1
trnV-trnY_p2	trnV-trnY	2	TCTGGCAAAGCAACAAGACT	TGTGGGCAGCACAGTTTATT	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.063181	152524	152577	943-947	1.000000	84.1
trnV-trnY_p3	trnV-trnY	3	ACGCAGTAGATCTGGCAAAG	ACAGGTGATGCAAGCTTTCA	20	20	50.0	45.0	58.0	58.0	5.2	0.1	0.030263	152524	152577	819-823	0.750000	72.8
trnV-trnY_p4	trnV-trnY	4	ACGCAGTAGATCTGGCAAAG	AATGTGGGCAGCACAGTTTA	20	20	50.0	45.0	58.0	58.0	5.2	0.0	0.021780	152524	152577	955-959	0.750000	72.6
trnV-trnY_p5	trnV-trnY	5	ACGCAGTAGATCTGGCAAAG	TGTGGGCAGCACAGTTTATT	20	20	50.0	45.0	58.0	58.0	5.2	0.0	0.021780	152524	152577	953-957	0.750000	72.6
trnY-trnT_p1	trnY-trnT	1	ACTGTAAATCCGCTGGCTTT	CTTCCACTGAGCTATAAGGGC	20	21	45.0	52.4	57.8	57.9	2.8	0.0	1.319278	152660	152680	89-93	1.000000	74.7
trnY-trnT_p2	trnY-trnT	2	GACTGTAAATCCGCTGGCTT	CTTCCACTGAGCTATAAGGGC	20	21	50.0	52.4	58.3	57.9	0.0	0.0	1.379838	152660	152680	90-94	1.000000	74.1
trnY-trnT_p3	trnY-trnT	3	ACTGTAAATCCGCTGGCTTT	TTCCACTGAGCTATAAGGGC	20	20	45.0	50.0	57.8	56.7	2.8	0.0	1.502637	152660	152680	88-92	1.000000	73.3
trnY-trnT_p4	trnY-trnT	4	GACTGTAAATCCGCTGGCTT	TTCCACTGAGCTATAAGGGC	20	20	50.0	50.0	58.3	56.7	0.0	0.0	1.563198	152660	152680	89-93	1.000000	72.7
trnY-trnT_p5	trnY-trnT	5	CTGTAAATCCGCTGGCTTTG	CTTCCACTGAGCTATAAGGGC	20	21	50.0	52.4	57.5	57.9	6.7	0.0	1.668538	152660	152680	88-92	1.000000	71.9