Hotspot

ndhH

SSC · 121264-122445 · 1182 bp

Back to Gelasia

Pi 0.00254

MarkerSeek score 60.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 3
pi: 0.00254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GCATCCATATGATCCCATAGACC	CGCTTTCAGCAATTCATGGA	47.8	45.0	58.3	57.1	1312 bp	1.0	46.5
ndhH_p2	GCATCCATATGATCCCATAGACC	TCGCTTTCAGCAATTCATGG	47.8	45.0	58.3	57.1	1313 bp	1.0	46.5
ndhH_p3	TCCATATGATCCCATAGACCTCT	CGCTTTCAGCAATTCATGGA	43.5	45.0	57.4	57.1	1309 bp	1.0	44.3
ndhH_p4	TCCATATGATCCCATAGACCTCT	TCGCTTTCAGCAATTCATGG	43.5	45.0	57.4	57.1	1310 bp	1.0	44.3
ndhH_p5	GCATCCATATGATCCCATAGACC	CGCTTTCAGCAATTCATGGAA	47.8	42.9	58.3	57.8	1312 bp	1.0	44.1