Hotspot

ndhF

SSC · 109150-111375 · 2226 bp

Back to Gelasia

Pi 0.0018

MarkerSeek score 43.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 4
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTAGCACTGGGTAGACCTCA	TCATTAGACCGATGACCCGA	55.0	50.0	57.9	57.9	2796 bp	1.0	78.6
ndhF_p2	CTAGCACTGGGTAGACCTCA	ATTAGACCGATGACCCGAGT	55.0	50.0	57.9	57.9	2794 bp	1.0	78.5
ndhF_p3	CTAGCACTGGGTAGACCTCA	CCGATGACCCGAGTTAATCC	55.0	55.0	57.9	57.8	2788 bp	1.0	77.9
ndhF_p4	CTAGCACTGGGTAGACCTCA	AGACCGATGACCCGAGTTAA	55.0	50.0	57.9	58.2	2791 bp	1.0	77.8
ndhF_p5	CTAGCACTGGGTAGACCTCA	TTAGACCGATGACCCGAGTT	55.0	50.0	57.9	58.2	2793 bp	1.0	77.8