Hotspot

ndhD

SSC · 114979-116508 · 1530 bp

Back to Gelasia

Pi 0

MarkerSeek score 26.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTAGTGATTCACGCGGTTCT	CTGCTGAAGCTGCTATTGGA	50.0	50.0	58.0	58.0	2366-2370 bp	1.0	79.8
ndhD_p2	GTAGTGATTCACGCGGTTCT	GCTGAAGCTGCTATTGGACT	50.0	50.0	58.0	58.0	2364-2368 bp	1.0	79.8
ndhD_p3	GTAGTGATTCACGCGGTTCT	AAAACTCGGGGAACTCATCG	50.0	50.0	58.0	57.9	2133-2137 bp	1.0	79.4
ndhD_p4	GTAGTGATTCACGCGGTTCT	GCCAAAACCTGTGCCAAAAT	50.0	45.0	58.0	58.0	1739 bp	0.5	59.7
ndhD_p5	CAGGGGGCTAATTCTGCAAT	GCCAAAACCTGTGCCAAAAT	50.0	45.0	57.9	58.0	1960 bp	0.5	59.1