Hotspot

ndhD-psaC

SSC · 116509-116599 · 91 bp

Back to Gelasia

Pi 0.0549

MarkerSeek score 81.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 91
variable_sites: 5
pi: 0.0549
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGGAACCCGCAAATATTGGT	ATTTCTTGAGCGTTCGGGTT	45.0	45.0	57.5	58.1	241-242 bp	1.0	76.6
ndhD-psaC_p2	AGGAACCCGCAAATATTGGT	ACAGAGGATTGTGTCGGTTG	45.0	50.0	57.5	57.8	290-291 bp	1.0	75.8
ndhD-psaC_p3	AGGAACCCGCAAATATTGGT	CAGAGGATTGTGTCGGTTGT	45.0	50.0	57.5	57.8	289-290 bp	1.0	75.8
ndhD-psaC_p4	AGGAACCCGCAAATATTGGT	TTTCTTGAGCGTTCGGGTTT	45.0	45.0	57.5	58.3	240-241 bp	1.0	74.8
ndhD-psaC_p5	AGGAACCCGCAAATATTGGT	AGAGATGTGAATCAGCCTGC	45.0	50.0	57.5	57.7	268-269 bp	1.0	74.7