Hotspot

rpl32-trnL-UAG

SSC · 112652-113561 · 910 bp

Back to Gelasia

Pi 0.00453
MarkerSeek score 62.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
910
variable_sites
4
pi
0.00453
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGATATTGGGCCGCCTTAAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 1009-1035 bp 1.0 82.3
rpl32-trnL-UAG_p2 GGATATTGGGCCGCCTTAAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 1010-1036 bp 1.0 82.3
rpl32-trnL-UAG_p3 GGATATTGGGCCGCCTTAAA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 1019-1045 bp 1.0 81.3
rpl32-trnL-UAG_p4 GGATATTGGGCCGCCTTAAA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.0 57.8 1018-1044 bp 1.0 80.8
rpl32-trnL-UAG_p5 GGATATTGGGCCGCCTTAAA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 1017-1043 bp 1.0 80.8