Hotspot

rpl22-rps19

LSC · 83807-83878 · 72 bp

Back to Gelasia

Pi 0.0161

MarkerSeek score 63.80

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 72
variable_sites: 1
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TTACTCTTCGCGCTTTGTGA	AAATTTCCGAGGACACGCAA	45.0	45.0	57.9	58.4	187-197 bp	1.0	80.1
rpl22-rps19_p2	TTACTCTTCGCGCTTTGTGA	AATTTCCGAGGACACGCAAA	45.0	45.0	57.9	58.4	186-196 bp	1.0	80.1
rpl22-rps19_p3	TTACTCTTCGCGCTTTGTGA	ATTTCCGAGGACACGCAAAA	45.0	45.0	57.9	58.4	185-195 bp	1.0	80.1
rpl22-rps19_p4	GAACGCCCACGAATTTGATC	ATTTCCGAGGACACGCAAAA	50.0	45.0	57.8	58.4	207-217 bp	1.0	79.6
rpl22-rps19_p5	GAACGCCCACGAATTTGATC	AATTTCCGAGGACACGCAAA	50.0	45.0	57.8	58.4	208-218 bp	1.0	79.5