Hotspot

psbB-psbT

LSC · 73893-74088 · 196 bp

Back to Gelasia

Pi 0.00541

MarkerSeek score 59.50

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 196
variable_sites: 1
pi: 0.00541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GGTGCTCGAACCTTGTTTAGA	TGAATTTTTGGCGGTTCTCT	47.6	40.0	58.2	55.9	394-405 bp	1.0	55.3
psbB-psbT_p2	GGTGCTCGAACCTTGTTTAG	TGAATTTTTGGCGGTTCTCT	50.0	40.0	56.7	55.9	394-405 bp	1.0	55.2
psbB-psbT_p3	GGTGCTCGAACCTTGTTTAGA	CCCTAGAGTCGAGACTAAGAGG	47.6	54.5	58.2	58.3	350-361 bp	1.0	54.7
psbB-psbT_p4	GGTGCTCGAACCTTGTTTAG	CCCTAGAGTCGAGACTAAGAGG	50.0	54.5	56.7	58.3	350-361 bp	1.0	54.6
psbB-psbT_p5	GGTGCTCGAACCTTGTTTAGA	TTGAATTTTTGGCGGTTCTCT	47.6	38.1	58.2	56.6	395-406 bp	1.0	53.5