Hotspot

clpP1-psbB

LSC · 71916-72365 · 450 bp

Back to Gelasia

Pi 0.00667

MarkerSeek score 59.50

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 3
pi: 0.00667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	ACCCAAGTTGCATCTTCCTC	TATGAACAGCAAGCAACCGT	50.0	45.0	57.8	58.1	582 bp	1.0	77.9
clpP1-psbB_p2	ACCCAAGTTGCATCTTCCTC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	532 bp	1.0	76.9
clpP1-psbB_p3	ACCCAAGTTGCATCTTCCTC	AGCAACCAAAGCTGTATGCA	50.0	45.0	57.8	58.4	605 bp	1.0	75.7
clpP1-psbB_p4	CCCAAGTTGCATCTTCCTCT	TATGAACAGCAAGCAACCGT	50.0	45.0	57.5	58.1	581 bp	1.0	75.5
clpP1-psbB_p5	ACCCAAGTTGCATCTTCCTC	ACACGATACCAAGGCAAACC	50.0	50.0	57.8	58.5	535 bp	1.0	75.0