Hotspot

psbE-petL

LSC · 64672-65900 · 1229 bp

Back to Gelasia

Pi 0.00163

MarkerSeek score 59.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 2
pi: 0.00163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCCGTCAGTAGAAAACCG	50.0	50.0	57.9	58.2	1376-1387 bp	1.0	78.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCCGTCAGTAGAAAACCGAA	50.0	50.0	57.9	58.2	1374-1385 bp	1.0	78.1
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCCGTCAGTAGAAAACCG	50.0	50.0	57.8	58.2	1299-1310 bp	1.0	77.3
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GCCGTCAGTAGAAAACCGAA	50.0	50.0	57.8	58.2	1297-1308 bp	1.0	77.3
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AAGCCGTCAGTAGAAAACCG	45.0	50.0	58.2	58.2	1385-1396 bp	1.0	77.2