Hotspot

trnY-GUA-rpoB

LSC · 11817-12907 · 1091 bp

Back to Gelasia

Pi 0.00278

MarkerSeek score 61.40

Primer available yes

Label trnY-GUA-rpoB

Feature Metadata

label_name: trnY-GUA-rpoB
feature_type: igs
region: LSC
length_bp: 1091
variable_sites: 3
pi: 0.00278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-rpoB_p1	CGAATTTACAGTCCGTCCCC	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.4	56.8	1202-1214 bp	1.0	60.5
trnY-GUA-rpoB_p2	ACGAATTTACAGTCCGTCCC	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	57.6	56.8	1203-1215 bp	1.0	60.3
trnY-GUA-rpoB_p3	CGAATTTACAGTCCGTCCCC	TCAAACCCTGATCAATGAACCT	55.0	40.9	58.4	57.6	1192-1204 bp	1.0	59.1
trnY-GUA-rpoB_p4	ACGAATTTACAGTCCGTCCC	TCAAACCCTGATCAATGAACCT	50.0	40.9	57.6	57.6	1193-1205 bp	1.0	58.9
trnY-GUA-rpoB_p5	CGAATTTACAGTCCGTCCCC	TCTTCTGTCAAACCCTGATCA	55.0	42.9	58.4	56.6	1199-1211 bp	1.0	58.8