Hotspot

ndhF

Genome · 141430-143667 · 2238 bp

Back to Gaultheria

Pi 0.0107

MarkerSeek score 49.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2238
variable_sites: 24
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGCTTCGAATCTAATTGCCA	ACAGAATAGTCAAGGGGCCT	40.0	50.0	55.4	58.0	2937-2950 bp	1.0	60.8
ndhF_p2	TGCTTCGAATCTAATTGCCA	TAGTCAAGGGGCCTTCTAGG	40.0	55.0	55.4	57.8	2931-2944 bp	1.0	59.6
ndhF_p3	TGCTTCGAATCTAATTGCCA	TCAAGGGGCCTTCTAGGATC	40.0	55.0	55.4	58.2	2928-2941 bp	1.0	59.4
ndhF_p4	TTGCTTCGAATCTAATTGCCA	ACAGAATAGTCAAGGGGCCT	38.1	50.0	56.2	58.0	2938-2951 bp	1.0	59.1
ndhF_p5	TGCTTCGAATCTAATTGCCA	TGCGCCCCTCTCCTATTAAT	40.0	50.0	55.4	58.3	2985-2998 bp	1.0	58.8