Hotspot

rpl32-ndhF

Genome · 139515-141429 · 1915 bp

Back to Gaultheria

Pi 0.0177

MarkerSeek score 61.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: Genome
length_bp: 1915
variable_sites: 33
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AGCTTTTAAGGCTGCCCAAT	AGACAGGAATGGGAAGTGGA	45.0	50.0	58.1	58.0	2024-2069 bp	1.0	82.4
rpl32-ndhF_p2	AAAGCTTTTAAGGCTGCCCA	AGACAGGAATGGGAAGTGGA	45.0	50.0	58.3	58.0	2026-2071 bp	1.0	80.6
rpl32-ndhF_p3	AAGCTTTTAAGGCTGCCCAA	AGACAGGAATGGGAAGTGGA	45.0	50.0	58.3	58.0	2025-2070 bp	1.0	80.6
rpl32-ndhF_p4	CTGCCCAATATCCTTTCCGT	AGACAGGAATGGGAAGTGGA	50.0	50.0	57.7	58.0	2013-2058 bp	1.0	80.1
rpl32-ndhF_p5	AGGCTGCCCAATATCCTTTC	AGACAGGAATGGGAAGTGGA	50.0	50.0	57.6	58.0	2016-2061 bp	1.0	79.9